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  • Effect of aqueous extract of Achillea millefolium on the development of experimental autoimmune encephalomyelitis in C57BL/6 mice

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    Author
    Vazirinejad, Reza
    Ayoobi, Fateme
    Arababadi, Mohammad Kazemi
    M. Eftekharian, Mohammad
    Darekordi, Ali
    Goudarzvand, Mahdi
    Hassanshahi, Gholamhossein
    Taghavi, Mohammad Mohsen
    Ahmadabadi, Behzad Nasiri
    Kennedy, Derek
    Shamsizadeh, Ali
    Year published
    2014
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    Abstract
    Objective: Achillea millefolium (A. millefolium) is widely used as an anti-inflammatory remedy in traditional and herbal medicine. In this study, we investigated the effect of an aqueous extract from A. millefolium on experimental autoimmune encephalomyelitis (EAE) and on the serum cytokine levels in C57BL/6 mice. Materials and Methods: EAE was induced in 63 C57BL/6 mice weighing 20-25 g (8 weeks old). Following immunization, the treatment protocol was initiated by using different doses of an aqueous extract from A. millefolium (1, 5, and 10 mg/mouse/day). Histopathologic assessments were performed by hematoxylin and eosin ...
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    Objective: Achillea millefolium (A. millefolium) is widely used as an anti-inflammatory remedy in traditional and herbal medicine. In this study, we investigated the effect of an aqueous extract from A. millefolium on experimental autoimmune encephalomyelitis (EAE) and on the serum cytokine levels in C57BL/6 mice. Materials and Methods: EAE was induced in 63 C57BL/6 mice weighing 20-25 g (8 weeks old). Following immunization, the treatment protocol was initiated by using different doses of an aqueous extract from A. millefolium (1, 5, and 10 mg/mouse/day). Histopathologic assessments were performed by hematoxylin and eosin (H and E) and luxol fast blue (LFB) staining. Behavioral disabilities were recorded by a camera. Serum levels of interleukin (IL)-10, IL-12, and transforming growth factor (TGF)-ߠwere measured using enzyme-linked immunosorbent assay (ELISA). Results: On average, mice developed classical behavioral disabilities of EAE, 13.2 ᠱ.9 days following immunization. Treatment of mice with A. millefolium led to delay the appearance of behavioral disabilities along with reduced severity of the behavioral disabilities. Treatment with A. millefolium prevented weight loss and increased serum levels of TGF-ߠin immunized mice with MOG35-55. EAE-induced mice, which were treated with A. millefolium, had less cerebral infiltration of inflammatory cells. Conclusion: The results demonstrated that treatment with aqueous extract of A. millefolium may attenuate disease severity, inflammatory responses, and demyelinating lesions in EAE-induced mice. In addition, following treatment with A. millefolium, serum levels of TGF-߷ere increased in EAE-induced mice.
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    Journal Title
    Indian Journal of Pharmacology
    Volume
    46
    Issue
    3
    Publisher URI
    http://www.ijp-online.com/
    Copyright Statement
    © 2014 Indian Association of Pathologists and Microbiologists. Published by Medknow. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
    Subject
    Central Nervous System
    Publication URI
    http://hdl.handle.net/10072/63894
    Collection
    • Journal articles

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