dc.contributor.author | Stanisic, Danielle | |
dc.contributor.author | Cutts, Julia | |
dc.contributor.author | Eriksson, E | |
dc.contributor.author | J. I. Fowkes, Freya | |
dc.contributor.author | Rosanas-Urgell, Anna | |
dc.contributor.author | Siba, Peter | |
dc.contributor.author | Laman, Moses | |
dc.contributor.author | M. E. Davis, Timothy | |
dc.contributor.author | Manning, Laurens | |
dc.contributor.author | Mueller, Ivo | |
dc.contributor.author | Schofield, Louis | |
dc.date.accessioned | 2018-08-02T22:00:48Z | |
dc.date.available | 2018-08-02T22:00:48Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 00221899 | |
dc.identifier.doi | 10.1093/infdis/jiu083 | |
dc.identifier.uri | http://hdl.handle.net/10072/64128 | |
dc.description.abstract | Background. Severe malaria (SM) is associated with high levels of cytokines such as tumor necrosis factor (TNF), interleukin 1 (IL-1), and interleukin 6 (IL-6). The role of chemokines is less clear, as is their cellular source. Methods. In a case-control study of children with SM (n = 200), uncomplicated malaria (UM) (n = 153) and healthy community controls (HC) (n = 162) in Papua, New Guinea, we measured cytokine/chemokine production by peripheral blood mononuclear cells (PBMCs) stimulated with live Plasmodium falciparum parasitized red blood cells (pRBC). Cellular sources were determined. Associations between immunological endpoints and clinical/parasitological variables were tested. Results. Compared to HC and UM, children with SM produced significantly higher IL-10, IP-10, MIP-1߭ and MCP-2. TNF and MIP-1a were significantly higher in the SM compared to the UM group. IL-10, IL-6, MIP-1a, MIP-1߬ and MCP-2 were associated with increased odds of SM. SM syndromes were associated with distinct cytokine/chemokine response profiles compared to UM cases. TNF, MIP-1߬ and MIP-1a were produced predominantly by monocytes and ?d T cells, and IL-10 by CD4+ T cells. Conclusions. Early/innate PBMC responses to pRBC in vitro are informative as to cytokines/chemokines associated with SM. Predominant cellular sources are monocytes and ?d T cells. Monocyte-derived chemokines support a role for monocyte infiltrates in the etiology of SM. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Oxford University Press | |
dc.publisher.place | United States | |
dc.relation.ispartofstudentpublication | N | |
dc.relation.ispartofpagefrom | 295 | |
dc.relation.ispartofpageto | 305 | |
dc.relation.ispartofissue | 2 | |
dc.relation.ispartofjournal | Journal of Infectious Diseases | |
dc.relation.ispartofvolume | 210 | |
dc.rights.retention | Y | |
dc.subject.fieldofresearch | Biological sciences | |
dc.subject.fieldofresearch | Biomedical and clinical sciences | |
dc.subject.fieldofresearch | Cellular immunology | |
dc.subject.fieldofresearchcode | 31 | |
dc.subject.fieldofresearchcode | 32 | |
dc.subject.fieldofresearchcode | 320404 | |
dc.title | γδ T cells and CD14+ Monocytes Are Predominant Cellular Sources of Cytokines and Chemokines Associated With Severe Malaria | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.faculty | Office of the Snr Dep Vice Chancellor, Institute for Glycomics | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Stanisic, Danielle | |