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dc.contributor.authorHart, Christopher
dc.contributor.authorIlanko, Pavithra
dc.contributor.authorP, Joseph
dc.contributor.authorRayan-Samuel, Paran
dc.contributor.authorMcDonnell, Ann
dc.contributor.authorCock, Ian
dc.date.accessioned2017-05-03T11:28:51Z
dc.date.available2017-05-03T11:28:51Z
dc.date.issued2014
dc.identifier.issn22490159
dc.identifier.urihttp://hdl.handle.net/10072/64210
dc.description.abstractIntroduction: Tasmannia stipitata (Dorrigo pepper) is an endemic Australian plant with a history of use by indigenous Australians as a food. It is taxonomically related to Tasmania lanceolata which has documented therapeutic properties as well as uses for food flavouring. Methods: T. stipitata solvent extracts were investigated by disc diffusion assay against a panel of bacteria and fungi. Their MIC values were determined to quantify and compare their efficacies. The ability to inhibit the proliferation of Giardia duodenalis was determined by direct cell counts and by using an MTS based cell proliferation assay. Toxicity was determined using the Artemia franciscana nauplii bioassay. Results: Methanolic, aqueous and ethyl acetate T. stipitata leaf and berry extracts displayed antibacterial activity in the disc diffusion assay. The berry methanolic extract had the broadest antibacterial range, inhibiting the growth of all 22 of the 23 bacteria tested (95.7%) and 2 of the 4 fungal species (50%) tested. In comparison, 18 of the bacterial species (81.8%) and 2 of the fungal species (50%) were inhibited by at least 1 of the leaf extracts. The methanol, water and ethyl acetate extracts of both berries and leaves all had similar efficacies and ranges of microbes inhibited. Whilst broad spectrum activity was seen for these extracts, they displayed only moderate to low efficacy (as determined by the zones of inhibition and MIC analyses). All extracts were more effective at inhibiting the growth Gram-negative bacteria than Gram-positive bacteria or fungi. Furthermore, the methanol, water and ethyl acetate extracts of both berry and leaf were potent inhibitors of Giardial proliferation. All T. stipitata extracts were non-toxic in the Artemia fransiscana bioassay with LC50 values greatly in excess of 1000 姯ml. Conclusion: The lack of toxicity of the T. stipitata extracts and their moderate broad spectrum inhibitory bioactivity against bacteria, fungi and Giardia indicates their potential as natural food preservatives and as medicinal agents in the treatment and prevention of microbial diseases.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent669352 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherPhcog.net
dc.publisher.placeIndia
dc.publisher.urihttp://www.phcogfirst.com/article/1225
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom33
dc.relation.ispartofpageto47
dc.relation.ispartofissue4
dc.relation.ispartofjournalPharmacognosy Communications
dc.relation.ispartofvolume4
dc.rights.retentionY
dc.subject.fieldofresearchComplementary and Alternative Medicine not elsewhere classified
dc.subject.fieldofresearchPlant Biology
dc.subject.fieldofresearchPharmacology and Pharmaceutical Sciences
dc.subject.fieldofresearchcode110499
dc.subject.fieldofresearchcode0607
dc.subject.fieldofresearchcode1115
dc.titleTasmannia stipitata as a Functional Food/Natural Preservative: Antimicrobial Activity and Toxicity
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2014 Phcog.net. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.date.issued2015-05-25T05:17:11Z
gro.hasfulltextFull Text
gro.griffith.authorCock, Ian E.
gro.griffith.authorMcDonnell, Ann A.
gro.griffith.authorRayan-Samuel, Paran
gro.griffith.authorP, Joseph S.
gro.griffith.authorHart, Christopher I.
gro.griffith.authorIlanko, Pavithra


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