Tasmannia lanceolata extracts: anti-Proteus activity and potential for the treatment and prevention of rheumatoid arthritis

View/ Open
Author(s)
Sirdaarta, Joseph
Winnett, Veronica
Matthews, B.
Cock, Ian Edwin
Year published
2014
Metadata
Show full item recordAbstract
Introduction. A wide variety of herbal medicines are used in indigenous Australian traditional medicinal systems to treat RA and inflammation. Tasmannia lanceolata (Tasmanian pepper) has received recent attention as a potential medicine due to its high antioxidant content (Cock 2013) and antibacterial activity (Winnet et al 2014).
Aims The current study was undertaken to test the ability of a panel of Tasmanian pepper extracts for the ability to block the microbial trigger of RA and to use metabolomics fingerprint analysis to detect anti-inflammatory compounds.
Methods. Tasmanian pepper berry and leaf were extracted with ...
View more >Introduction. A wide variety of herbal medicines are used in indigenous Australian traditional medicinal systems to treat RA and inflammation. Tasmannia lanceolata (Tasmanian pepper) has received recent attention as a potential medicine due to its high antioxidant content (Cock 2013) and antibacterial activity (Winnet et al 2014). Aims The current study was undertaken to test the ability of a panel of Tasmanian pepper extracts for the ability to block the microbial trigger of RA and to use metabolomics fingerprint analysis to detect anti-inflammatory compounds. Methods. Tasmanian pepper berry and leaf were extracted with solvents of varying polarity and investigated for the ability to inhibit the growth of the bacterial trigger of RA (P. mirabilis). The extracts were tested for toxicity in the Artemia nauplii bioassay. The most potent inhibitor of P. mirabilis growth was further analysed by RP-HPLC coupled to high accuracy TOF mass spectroscopy. Results. The Tasmanian pepper berry extracts were determined to be the most effective inhibitors of P. mirabilis growth, with MIC values as low as 11 and 126 µg/ml for the methanolic and aqueous extracts respectively. Subsequent analysis of the T. lanceolata fruit extracts by RP-HPLC coupled to high resolution TOF mass spectroscopy detected the resveratrol glycoside piceid and 2 combretastatin stilbenes in both T. lanceolata fruit extracts. All extracts were also shown to be non-toxic in the Artemia nauplii bioassay. Discussion. The low toxicity of these extracts and their inhibitory bioactivity against Proteus spp. indicate their potential in blocking the onset of rheumatoid arthritis.
View less >
View more >Introduction. A wide variety of herbal medicines are used in indigenous Australian traditional medicinal systems to treat RA and inflammation. Tasmannia lanceolata (Tasmanian pepper) has received recent attention as a potential medicine due to its high antioxidant content (Cock 2013) and antibacterial activity (Winnet et al 2014). Aims The current study was undertaken to test the ability of a panel of Tasmanian pepper extracts for the ability to block the microbial trigger of RA and to use metabolomics fingerprint analysis to detect anti-inflammatory compounds. Methods. Tasmanian pepper berry and leaf were extracted with solvents of varying polarity and investigated for the ability to inhibit the growth of the bacterial trigger of RA (P. mirabilis). The extracts were tested for toxicity in the Artemia nauplii bioassay. The most potent inhibitor of P. mirabilis growth was further analysed by RP-HPLC coupled to high accuracy TOF mass spectroscopy. Results. The Tasmanian pepper berry extracts were determined to be the most effective inhibitors of P. mirabilis growth, with MIC values as low as 11 and 126 µg/ml for the methanolic and aqueous extracts respectively. Subsequent analysis of the T. lanceolata fruit extracts by RP-HPLC coupled to high resolution TOF mass spectroscopy detected the resveratrol glycoside piceid and 2 combretastatin stilbenes in both T. lanceolata fruit extracts. All extracts were also shown to be non-toxic in the Artemia nauplii bioassay. Discussion. The low toxicity of these extracts and their inhibitory bioactivity against Proteus spp. indicate their potential in blocking the onset of rheumatoid arthritis.
View less >
Conference Title
ASCEPT 2014
Publisher URI
Copyright Statement
© The Author(s) 2014. The attached file is reproduced here in accordance with the copyright policy of the publisher. For information about this conference please refer to the conference’s website or contact the authors.
Subject
Pharmaceutical Sciences