Characterization of microsatellite loci in the straw-colored fruit bat, Eidolon helvum (Pteropodidae)

Peel, Alison; Rossiter, Stephen; Wood, James; Cunningham, Andrew; Sargan, David

doi:10.1007/s12686-010-9190-9

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2010

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Abstract

Twenty microsatellite loci developed for the straw-colored fruit bat, Eidolon helvum, are described. These markers were used in multiplex PCRs to amplify genomic DNA from 142 individuals sampled from nine populations across Africa. Nineteen loci were polymorphic, with a mean number of alleles per locus of 16.2 (3-46). Observed and expected heterozygosity values ranged from 0.01 to 0.90 and 0.03 to 0.97, respectively. These markers will be used in combination with mitochondrial DNA sequences to investigate gene flow and the genetic metapopulation structure of E. helvum.Twenty microsatellite loci developed for the straw-colored fruit bat, Eidolon helvum, are described. These markers were used in multiplex PCRs to amplify genomic DNA from 142 individuals sampled from nine populations across Africa. Nineteen loci were polymorphic, with a mean number of alleles per locus of 16.2 (3-46). Observed and expected heterozygosity values ranged from 0.01 to 0.90 and 0.03 to 0.97, respectively. These markers will be used in combination with mitochondrial DNA sequences to investigate gene flow and the genetic metapopulation structure of E. helvum.
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Journal Title

Conservation Genetic Resources

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DOI

https://doi.org/10.1007/s12686-010-9190-9

Copyright Statement

© 2010 Springer Netherlands. This is an electronic version of an article published in Conservation Genetic Resources, Vol. 2(1), 2010, pp. 279-282. Conservation Genetic Resources is available online at: http://link.springer.com/ with the open URL of your article.

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Genetics not elsewhere classified

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http://hdl.handle.net/10072/64701

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Journal articles