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  • Characterization of microsatellite loci in the straw-colored fruit bat, Eidolon helvum (Pteropodidae)

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    Author(s)
    Peel, Alison J
    Rossiter, Stephen J
    Wood, James LN
    Cunningham, Andrew A
    Sargan, David R
    Griffith University Author(s)
    Peel, Alison J.
    Year published
    2010
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    Abstract
    Twenty microsatellite loci developed for the straw-colored fruit bat, Eidolon helvum, are described. These markers were used in multiplex PCRs to amplify genomic DNA from 142 individuals sampled from nine populations across Africa. Nineteen loci were polymorphic, with a mean number of alleles per locus of 16.2 (3-46). Observed and expected heterozygosity values ranged from 0.01 to 0.90 and 0.03 to 0.97, respectively. These markers will be used in combination with mitochondrial DNA sequences to investigate gene flow and the genetic metapopulation structure of E. helvum.Twenty microsatellite loci developed for the straw-colored fruit bat, Eidolon helvum, are described. These markers were used in multiplex PCRs to amplify genomic DNA from 142 individuals sampled from nine populations across Africa. Nineteen loci were polymorphic, with a mean number of alleles per locus of 16.2 (3-46). Observed and expected heterozygosity values ranged from 0.01 to 0.90 and 0.03 to 0.97, respectively. These markers will be used in combination with mitochondrial DNA sequences to investigate gene flow and the genetic metapopulation structure of E. helvum.
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    Journal Title
    Conservation Genetic Resources
    Volume
    2
    Issue
    1
    DOI
    https://doi.org/10.1007/s12686-010-9190-9
    Copyright Statement
    © 2010 Springer Netherlands. This is an electronic version of an article published in Conservation Genetic Resources, Vol. 2(1), 2010, pp. 279-282. Conservation Genetic Resources is available online at: http://link.springer.com/ with the open URL of your article.
    Subject
    Evolutionary biology
    Genetics
    Genetics not elsewhere classified
    Fisheries sciences
    Publication URI
    http://hdl.handle.net/10072/64701
    Collection
    • Journal articles

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