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dc.contributor.authorGassling, Volkeren_US
dc.contributor.authorDouglas, Timothyen_US
dc.contributor.authorH. Warnke, Patricken_US
dc.contributor.authorAcil, Yahyaen_US
dc.contributor.authorWiltfang, Jorgen_US
dc.contributor.authorT. Becker, Stephanen_US
dc.date.accessioned2017-05-03T14:41:46Z
dc.date.available2017-05-03T14:41:46Z
dc.date.issued2010en_US
dc.identifier.issn09057161en_US
dc.identifier.doi10.1111/j.1600-0501.2009.01900.xen_US
dc.identifier.urihttp://hdl.handle.net/10072/64712
dc.description.abstractObjectives: Platelet-rich fibrin (PRF)-based membranes have been used for covering alveolar ridge augmentation side in several in vivo studies. Few in vitro studies on PRF and no studies using human periosteal cells for tissue engineering have been published. The aim is a comparison of PRF with the commonly used collagen membrane Bio-Gides scaffolds for periosteal tissue engineering. Material and methods: Human periosteal cells were seeded on membrane pieces (collagen [Bio-Gideand PRF) at a density of 104 cells/well. Cell vitality was assessed by fluorescein diacetate (FDA) and propidium iodide (PI) staining, biocompatibility with the lactate dehydrogenase (LDH) test and proliferation level with the MTT, WST and BrdU tests and scanning electron microscopy (SEM). Results: PRF membranes showed slightly inferior biocompatibility, as shown by the LDH test. The metabolic activity measured by the MTT and WST tests was higher for PRF than for collagen (BioGide The proliferation level as measured by the BrdU test (quantitative) and SEM examinations (qualitative) revealed higher values for PRF. Conclusion: PRF appears to be superior to collagen (Bio-Gideas a scaffold for human periosteal cell proliferation. PRF membranes are suitable for in vitro cultivation of periosteal cells for bone tissue engineering.en_US
dc.description.peerreviewedYesen_US
dc.description.publicationstatusYesen_US
dc.languageEnglishen_US
dc.publisherWiley-Blackwell Publishing, Inc.en_US
dc.publisher.placeUnited Statesen_US
dc.relation.ispartofstudentpublicationNen_US
dc.relation.ispartofpagefrom543en_US
dc.relation.ispartofpageto549en_US
dc.relation.ispartofissue5en_US
dc.relation.ispartofjournalClinical Oral Implants Researchen_US
dc.relation.ispartofvolume21en_US
dc.rights.retentionYen_US
dc.subject.fieldofresearchDentistry not elsewhere classifieden_US
dc.subject.fieldofresearchcode110599en_US
dc.titlePlatelet-rich fibrin membranes as scaffolds for periosteal tissue engineeringen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.hasfulltextNo Full Text
gro.griffith.authorWarnke, Patrick H.


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