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  • Bioactive and osteoblast cell attachment studies of novel α- and β-chitin membranes for tissue-engineering applications

    Author(s)
    Jayakumar, R.
    Divya Rani, V.
    Shalumon, K.
    Sudheesh Kumar, P.
    Nair, S.
    Furuike, T.
    Tamura, H.
    Griffith University Author(s)
    Pandiyan Thodi, Sudheesh Kumar
    Year published
    2009
    Metadata
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    Abstract
    Chitin is a novel biopolymer and has excellent biological properties such as biodegradation in the human body and biocompatible, bioabsorable, antibacterial and wound healing activities. In this work, a- and ߭chitin membranes were prepared using a- and ߭chitin hydrogel. The bioactivity studies were carried out using these chitin membranes with the simulated body fluid solution (SBF) for 7, 14 and 21 days. After 7, 14 and 21 days the membranes were characterized using SEM, EDS and FT-IR. The SEM, EDS and FT-IR studies confirmed the formation of calcium phosphate layer on the surface of the both chitin membranes. These results ...
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    Chitin is a novel biopolymer and has excellent biological properties such as biodegradation in the human body and biocompatible, bioabsorable, antibacterial and wound healing activities. In this work, a- and ߭chitin membranes were prepared using a- and ߭chitin hydrogel. The bioactivity studies were carried out using these chitin membranes with the simulated body fluid solution (SBF) for 7, 14 and 21 days. After 7, 14 and 21 days the membranes were characterized using SEM, EDS and FT-IR. The SEM, EDS and FT-IR studies confirmed the formation of calcium phosphate layer on the surface of the both chitin membranes. These results indicate that the prepared chitin membranes were bioactive. Cell adhesion studies were also carried out using MG-63 osteoblast-like cells. The cells were adhered and spread over the membrane after 24 h of incubation. These results indicated that the chitin membranes could be used for tissue-engineering applications.
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    Journal Title
    International Journal of Biological Macromolecules
    Volume
    45
    Issue
    3
    DOI
    https://doi.org/10.1016/j.ijbiomac.2009.06.002
    Subject
    Biochemistry and Cell Biology not elsewhere classified
    Biochemistry and Cell Biology
    Publication URI
    http://hdl.handle.net/10072/64730
    Collection
    • Journal articles

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