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dc.contributor.authorT. Becker, Stephan
dc.contributor.authorDouglas, Timothy
dc.contributor.authorAcil, Yahya
dc.contributor.authorSeitz, Hermann
dc.contributor.authorSivananthan, Sureshan
dc.contributor.authorWiltfang, Jorg
dc.contributor.authorH. Warnke, Patrick
dc.date.accessioned2017-05-03T14:41:47Z
dc.date.available2017-05-03T14:41:47Z
dc.date.issued2010
dc.identifier.issn09574530
dc.identifier.doi10.1007/s10856-009-3878-y
dc.identifier.urihttp://hdl.handle.net/10072/64732
dc.description.abstractThe aim of this study was to evaluate and compare the biocompatibility of computer-assisted designed (CAD) synthetic hydroxyapatite (HA) and tricalciumphosphate (TCP) blocks and natural bovine hydroxyapatite blocks for augmentations and endocultivation by supporting and promoting the proliferation of human periosteal cells. Human periosteum cells were cultured using an osteogenic medium consisting of Dulbecco's modified Eagle medium supplemented with fetal calf serum, Penicillin, Streptomycin and ascorbic acid at 37àwith 5% CO2. Three scaffolds were tested: 3D-printed HA, 3D-printed TCP and bovine HA. Cell vitality was assessed by Fluorescein Diacetate (FDA) and Propidium Iodide (PI) staining, biocompatibility with LDH, MTT, WST and BrdU tests, and scanning electron microscopy. Data were analyzed with ANOVAs. Results: After 24 h all samples showed viable periosteal cells, mixed with some dead cells for the bovine HA group and very few dead cells for the printed HA and TCP groups. The biocompatibility tests revealed that proliferation on all scaffolds after treatment with eluate was sometimes even higher than controls. Scanning electron microscopy showed that periosteal cells formed layers covering the surfaces of all scaffolds 7 days after seeding. Conclusion: It can be concluded from our data that the tested materials are biocompatible for periosteal cells and thus can be used as scaffolds to augment bone using tissue engineering methods.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer New York LLC
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1255
dc.relation.ispartofpageto1262
dc.relation.ispartofissue4
dc.relation.ispartofjournalJournal of Materials Science: Materials in Medicine
dc.relation.ispartofvolume21
dc.rights.retentionY
dc.subject.fieldofresearchDentistry not elsewhere classified
dc.subject.fieldofresearchBiomedical Engineering
dc.subject.fieldofresearchMaterials Engineering
dc.subject.fieldofresearchcode110599
dc.subject.fieldofresearchcode0903
dc.subject.fieldofresearchcode0912
dc.titleBiocompatibility of individually designed scaffolds with human periosteum for use in tissue engineering
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorWarnke, Patrick H.


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