Show simple item record

dc.contributor.authorC. Dash, Biraja
dc.contributor.authorMahor, Sunil
dc.contributor.authorCarroll, Oliver
dc.contributor.authorMathew, Asha
dc.contributor.authorWang, Wenxin
dc.contributor.authorA. Woodhouse, Kimberly
dc.contributor.authorPandit, Abhay
dc.date.accessioned2017-05-03T12:45:24Z
dc.date.available2017-05-03T12:45:24Z
dc.date.issued2011
dc.identifier.issn01683659
dc.identifier.doi10.1016/j.jconrel.2011.03.006
dc.identifier.urihttp://hdl.handle.net/10072/64738
dc.description.abstractSelf-assembly driven processes can be utilized to produce a variety of nanostructures useful for various in vitro and in vivo applications. Characteristics such as size, stability, biocompatibility, high therapeutic loading and controlled delivery of these nanostructures are particularly crucial in relation to in vivo applications. In this study, we report the fabrication of tunable monodispersed elastin-like polypeptide (ELP) hollow spheres of 100, 300, 500 and 1000 nm by exploiting the self-assembly property and net positive charge of ELP. The microbial transglutaminase (mTGase) cross-linking provided robustness and stability to the hollow spheres while maintaining surface functional groups for further modifications. The resulting hollow spheres showed a higher loading efficiency of plasmid DNA (pDNA) by using polyplex (~ 70 姠pDNA/mg of hollow sphere) than that of self-assembled ELP particles and demonstrated controlled release triggered by protease and elastase. Moreover, polyplex-loaded hollow spheres showed better cell viability than polyplex alone and yielded higher luciferase expression by providing protection against endosomal degradation. Overall, the monodispersed, tunable hollow spheres with a capability of post-functionalization can provide an exciting new opportunity for use in a range of therapeutic and diagnostic applications.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.publisher.placeNetherlands
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom382
dc.relation.ispartofpageto392
dc.relation.ispartofissue3
dc.relation.ispartofjournalJournal of Controlled Release
dc.relation.ispartofvolume152
dc.rights.retentionY
dc.subject.fieldofresearchBiomedical Engineering not elsewhere classified
dc.subject.fieldofresearchPharmacology and Pharmaceutical Sciences not elsewhere classified
dc.subject.fieldofresearchBiomedical Engineering
dc.subject.fieldofresearchChemical Engineering
dc.subject.fieldofresearchPharmacology and Pharmaceutical Sciences
dc.subject.fieldofresearchcode090399
dc.subject.fieldofresearchcode111599
dc.subject.fieldofresearchcode0903
dc.subject.fieldofresearchcode0904
dc.subject.fieldofresearchcode1115
dc.titleTunable elastin-like polypeptide hollow sphere as a high payload and controlled delivery gene depot
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorMathew, Asha


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record