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  • JK1 (FAM134B) represses cell migration in colon cancer: a functional study of a novel gene

    Author(s)
    Kasem, Kais
    Sullivan, Emily
    Gopalan, Vinod
    Salajegheh, Ali
    Smith, Robert A
    Lam, Alfred K-Y
    Griffith University Author(s)
    Lam, Alfred K.
    Ariana, Armin S.
    Gopalan, Vinod
    Year published
    2014
    Metadata
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    Abstract
    Background JK1 is a novel cancer-related gene with unknown functional role in carcinogenesis. The aim of this study is to investigate the role of JK1 gene in carcinogenesis in an in vitro cell proliferation and migration analysis model. Methods Small hairpin RNAs (shRNA) were designed to knock-down JK1 expression in colon cancer cell line (SW480) using transduction ready lentiviral particles. Cell proliferation and cell migration assays were performed on multiple extracellular matrices to investigate the cellular effects of JK1 in colon cancer cells. A non-cancer colonic epithelial cell line (FHC) was used to compare the ...
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    Background JK1 is a novel cancer-related gene with unknown functional role in carcinogenesis. The aim of this study is to investigate the role of JK1 gene in carcinogenesis in an in vitro cell proliferation and migration analysis model. Methods Small hairpin RNAs (shRNA) were designed to knock-down JK1 expression in colon cancer cell line (SW480) using transduction ready lentiviral particles. Cell proliferation and cell migration assays were performed on multiple extracellular matrices to investigate the cellular effects of JK1 in colon cancer cells. A non-cancer colonic epithelial cell line (FHC) was used to compare the expression of JK1 in cancer cell line. Results JK1 knock-down did not affect cellular proliferation or survival in colon cancer. However, the manipulation increased cancer cell migration rates on collagen and fibronectin substrates. Conclusions JK1 was shown for the first time to have a functional role in the pathogenesis of colon cancer. The results imply that JK1 represses the capacity of cancer cells to migrate within their tissue. They also concurred with the previous findings of JK1 activity correlations with clinical and pathological features in colon cancer. The capacity may have utility as a means to prevent cancer cells forming metastases.
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    Journal Title
    Experimental and Molecular Pathology
    Volume
    97
    Issue
    1
    DOI
    https://doi.org/10.1016/j.yexmp.2014.06.002
    Subject
    Clinical sciences
    Publication URI
    http://hdl.handle.net/10072/64750
    Collection
    • Journal articles

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