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dc.contributor.authorKasem, Kais
dc.contributor.authorSullivan, Emily
dc.contributor.authorGopalan, Vinod
dc.contributor.authorSalajegheh, Ali
dc.contributor.authorSmith, Robert A
dc.contributor.authorLam, Alfred K-Y
dc.description.abstractBackground JK1 is a novel cancer-related gene with unknown functional role in carcinogenesis. The aim of this study is to investigate the role of JK1 gene in carcinogenesis in an in vitro cell proliferation and migration analysis model. Methods Small hairpin RNAs (shRNA) were designed to knock-down JK1 expression in colon cancer cell line (SW480) using transduction ready lentiviral particles. Cell proliferation and cell migration assays were performed on multiple extracellular matrices to investigate the cellular effects of JK1 in colon cancer cells. A non-cancer colonic epithelial cell line (FHC) was used to compare the expression of JK1 in cancer cell line. Results JK1 knock-down did not affect cellular proliferation or survival in colon cancer. However, the manipulation increased cancer cell migration rates on collagen and fibronectin substrates. Conclusions JK1 was shown for the first time to have a functional role in the pathogenesis of colon cancer. The results imply that JK1 represses the capacity of cancer cells to migrate within their tissue. They also concurred with the previous findings of JK1 activity correlations with clinical and pathological features in colon cancer. The capacity may have utility as a means to prevent cancer cells forming metastases.
dc.publisherAcademic Press
dc.publisher.placeUnited States
dc.relation.ispartofjournalExperimental and Molecular Pathology
dc.subject.fieldofresearchMedical and Health Sciences not elsewhere classified
dc.subject.fieldofresearchClinical Sciences
dc.titleJK1 (FAM134B) represses cell migration in colon cancer: a functional study of a novel gene
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Medicine
gro.hasfulltextNo Full Text
gro.griffith.authorSmith, Robert A.
gro.griffith.authorSullivan, Emily M.
gro.griffith.authorLam, Alfred K.
gro.griffith.authorAriana, Armin S.
gro.griffith.authorGopalan, Vinod
gro.griffith.authorKasem, Kais Y.

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