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dc.contributor.authorKasem, Kaisen_US
dc.contributor.authorSullivan, Emilyen_US
dc.contributor.authorGopalan, Vinoden_US
dc.contributor.authorAriana, Arminen_US
dc.contributor.authorSmith, Roberten_US
dc.contributor.authorLam, Alfreden_US
dc.description.abstractBackground JK1 is a novel cancer-related gene with unknown functional role in carcinogenesis. The aim of this study is to investigate the role of JK1 gene in carcinogenesis in an in vitro cell proliferation and migration analysis model. Methods Small hairpin RNAs (shRNA) were designed to knock-down JK1 expression in colon cancer cell line (SW480) using transduction ready lentiviral particles. Cell proliferation and cell migration assays were performed on multiple extracellular matrices to investigate the cellular effects of JK1 in colon cancer cells. A non-cancer colonic epithelial cell line (FHC) was used to compare the expression of JK1 in cancer cell line. Results JK1 knock-down did not affect cellular proliferation or survival in colon cancer. However, the manipulation increased cancer cell migration rates on collagen and fibronectin substrates. Conclusions JK1 was shown for the first time to have a functional role in the pathogenesis of colon cancer. The results imply that JK1 represses the capacity of cancer cells to migrate within their tissue. They also concurred with the previous findings of JK1 activity correlations with clinical and pathological features in colon cancer. The capacity may have utility as a means to prevent cancer cells forming metastases.en_US
dc.publisherAcademic Pressen_US
dc.publisher.placeUnited Statesen_US
dc.relation.ispartofjournalExperimental and Molecular Pathologyen_US
dc.subject.fieldofresearchMedical and Health Sciences not elsewhere classifieden_US
dc.titleJK1 (FAM134B) represses cell migration in colon cancer: a functional study of a novel geneen_US
dc.typeJournal articleen_US
dc.type.descriptionC1 - Peer Reviewed (HERDC)en_US
dc.type.codeC - Journal Articlesen_US
gro.facultyGriffith Health, School of Medicineen_US
gro.hasfulltextNo Full Text

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