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dc.contributor.authorSudheesh Kumar, P.
dc.contributor.authorMincy Raj, N.
dc.contributor.authorPraveen, G.
dc.contributor.authorChennazhi, Krishna Prasad
dc.contributor.authorV. Nair, Shantikumar
dc.contributor.authorJayakumar, R.
dc.date.accessioned2017-05-03T12:45:26Z
dc.date.available2017-05-03T12:45:26Z
dc.date.issued2013
dc.identifier.issn19373341
dc.identifier.doi10.1089/ten.tea.2012.0376
dc.identifier.urihttp://hdl.handle.net/10072/64882
dc.description.abstractIn this work, we have developed chitosan hydrogel/nanofibrin composite bandages (CFBs) and characterized using Fourier transform-infrared spectroscopy and scanning electron microscopy. The homogeneous distribution of nanofibrin in the prepared chitosan hydrogel matrix was confirmed by phosphotungstic acid-hematoxylin staining. The mechanical strength, swelling, biodegradation, porosity, whole-blood clotting, and platelet activation studies were carried out. In addition, the cell viability, cell attachment, and infiltration of the prepared CFBs were evaluated using human umbilical vein endothelial cells (HUVECs) and human dermal fibroblast (HDF) cells. It was found that the CFBs were microporous, flexible, biodegradable, and showed enhanced blood clotting and platelet activity compared to the one without nanofibrin. The prepared CFBs were capable of absorbing fluid and this was confirmed when immersed in phosphate buffered saline. Cell viability studies on HUVECs and HDF cells proved the nontoxic nature of the CFBs. Cell attachment and infiltration studies showed that the cells were found attached and proliferated on the CFBs. In vivo experiments were carried out in Sprague-Dawley rats and found that the wound healing occurred within 2 weeks when treated with CFBs than compared to the bare wound and wound treated with Kaltostat. The deposition of collagen was found to be more on CFB-treated wounds compared to the control. The above results proved the use of these CFBs as an ideal candidate for skin tissue regeneration and wound healing.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherMary Ann Liebert, Inc. Publishers
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom380
dc.relation.ispartofpageto392
dc.relation.ispartofissue3-4
dc.relation.ispartofjournalTissue Engineering. Part A
dc.relation.ispartofvolume19
dc.rights.retentionY
dc.subject.fieldofresearchBiochemistry and Cell Biology not elsewhere classified
dc.subject.fieldofresearchBiomedical Engineering not elsewhere classified
dc.subject.fieldofresearchBiochemistry and Cell Biology
dc.subject.fieldofresearchBiomedical Engineering
dc.subject.fieldofresearchMaterials Engineering
dc.subject.fieldofresearchcode060199
dc.subject.fieldofresearchcode090399
dc.subject.fieldofresearchcode0601
dc.subject.fieldofresearchcode0903
dc.subject.fieldofresearchcode0912
dc.titleIn vitro and in vivo evaluation of microporous chitosan hydrogel/nanofibrin composite bandage for skin tissue regeneration
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorPandiyan Thodi, Sudheesh Kumar


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