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dc.contributor.authorJiang, Yuelu
dc.contributor.authorYin, Kedong
dc.contributor.authorBerges, John A
dc.contributor.authorHarrison, Paul J
dc.date.accessioned2019-04-11T05:31:21Z
dc.date.available2019-04-11T05:31:21Z
dc.date.issued2014
dc.identifier.issn1529-8817
dc.identifier.doi10.1111/jpy.12176
dc.identifier.urihttp://hdl.handle.net/10072/64971
dc.description.abstractThe ability of nutrient‐deprived phytoplankton to recover in the short term when nutrients are resupplied has been studied for nitrogen and phosphorus, but the case for silicate (Si) is poorly understood. Si‐limited Thalassiosira weissflogii (Grunow) Fryxell et Hasle (grown in batch culture) was harvested in stationary phase (when cell numbers stopped increasing ~2 d after Si depletion) and senescence (when cell numbers declined ~4 d after Si depletion) and Si was resupplied at different concentrations (from 0 to 100 μM). Cell numbers, proportion of dead cells, variable fluorescence emissions (Fv/Fm), and activities of proteases were measured during Si depletion and for 24 h after Si resupply. As Si was depleted, the specific growth rate declined, dead cells increased from ~2% in log phase, to ~25% in stationary phase to over 35% in senescence, and activities of proteases associated with cell death increased several‐fold. Concentration‐dependent recovery of growth rate was seen after 24 h for cultures resupplied with Si in stationary phase but not in senescence. However, resupply of Si at 100 μM to stationary phase cultures alone increased protease activity to nearly the levels seen in senescence. Differences in the responses to Si resupply suggest that the ability and time to recover from Si depletion depend not only on the growth phase but also on the concentration resupplied.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageeng
dc.publisherWiley-Blackwell
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom602
dc.relation.ispartofpageto606
dc.relation.ispartofissue3
dc.relation.ispartofjournalJournal of Phycology
dc.relation.ispartofvolume50
dc.rights.retentionY
dc.subject.fieldofresearchPlant Biology not elsewhere classified
dc.subject.fieldofresearchPlant Biology
dc.subject.fieldofresearchcode060799
dc.subject.fieldofresearchcode0607
dc.titleEffects of silicate resupply to silicate-deprived thalassiosira weissflogii (bacillariophyta) in stationary or senescent phase: Short-term patterns of growth and cell death
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyrightSelf-archiving of the author-manuscript version is not yet supported by this journal. Please refer to the journal link for access to the definitive, published version or contact the authors for more information.
gro.hasfulltextNo Full Text
gro.griffith.authorYin, Kedong


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