Show simple item record

dc.contributor.authorZhang, L
dc.contributor.authorGowardman, J
dc.contributor.authorMorrison, M
dc.contributor.authorKrause, L
dc.contributor.authorPlayford, EG
dc.contributor.authorRickard, CM
dc.date.accessioned2017-10-05T03:50:02Z
dc.date.available2017-10-05T03:50:02Z
dc.date.issued2014
dc.identifier.issn0934-9723
dc.identifier.doi10.1007/s10096-014-2058-2
dc.identifier.urihttp://hdl.handle.net/10072/65084
dc.description.abstractIntravascular catheter-related bloodstream infections (IVC-BSIs) are associated with significant morbidity and mortality. Culture-independent molecular approaches can reveal and capture the composition of complex microbial communities, and are now being used to reveal "new" pathogens as well as the polymicrobial nature of some infections. Patients with concurrently sited arterial and central venous catheters who had clinically suspected IVC-BSIs, were examined by the high-throughput sequencing of microbial 16S rRNA. An average of 100 operational taxonomic units (OTUs, phylotypes) was observed on each IVC, indicating that IVCs were colonised by complex and diverse bacterial communities. Ralstonia (53 % of 16S rRNA sequences), Escherichia group (16 %), Propionibacterium (5 %), Staphylococcus (5 %), and Streptococcus (2 %) were the most abundant genera. There was no statistically significant difference in the bacterial communities examined from arterial and central venous catheters; from those with and without systemic antibiotic treatment; or from conventionally colonised and uncolonised IVCs. The genome of the predominant bacteria, R. pickettii AU12-08, was found to encode resistance to antimicrobial drugs of different classes. In addition, many encoded gene products are involved in quorum sensing and biofilm formation that would further contribute to increased antimicrobial drug resistance. Our results highlight the complex diversity of microbial ecosystems on vascular devices. High-throughput sequencing of 16S rRNA offers an insight into the pathogenesis of IVC-related infections, and opens up the scope for improving diagnosis and patient management.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherSpringer
dc.publisher.placeGermany
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1189
dc.relation.ispartofpageto1198
dc.relation.ispartofissue7
dc.relation.ispartofjournalEuropean Journal of Clinical Microbiology & Infectious Diseases
dc.relation.ispartofvolume33
dc.rights.retentionY
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchBiomedical and clinical sciences
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode32
dc.titleMolecular investigation of bacterial communities on intravascular catheters: No longer just Staphylococcus
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionAccepted Manuscript (AM)
gro.facultyGriffith Health, School of Nursing and Midwifery
gro.rights.copyright© 2014 Springer Berlin / Heidelberg. This is an electronic version of an article published in European Journal of Clinical Microbiology & Infectious Diseases, Vol. 33(7), pp. 1189-1198, 2014. European Journal of Clinical Microbiology & Infectious Diseases is available online at: http://link.springer.com/ with the open URL of your article.
gro.hasfulltextFull Text
gro.griffith.authorZhang, Li
gro.griffith.authorGowardman, John R.
gro.griffith.authorRickard, Catherine M.
gro.griffith.authorRickard, Claire
gro.griffith.authorPlayford, Elliott G.


Files in this item

This item appears in the following Collection(s)

  • Journal articles
    Contains articles published by Griffith authors in scholarly journals.

Show simple item record