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  • Regulation of microRNA-1288 in colorectal cancer: Altered expression and its clinicopathological significance

    Author(s)
    Gopalan, Vinod
    Pillai, Suja
    Ebrahimi, Faeza
    Salajegheh, Ali
    Lam, Tommy C
    Le, Tran K
    Langsford, Nicole
    Ho, Yik-Hong
    Smith, Robert A
    Lam, Alfred K-Y
    Griffith University Author(s)
    Lam, Alfred K.
    Ariana, Armin S.
    Gopalan, Vinod
    Year published
    2014
    Metadata
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    Abstract
    We aim to examine the miR-1288 expression in cancer cell lines and a large cohort of patients with colorectal cancer. Two colon cancer cell lines (SW480 and SW48) and one normal colonic epithelial cell line (FHC) were recruited. The miRNA expressions of miR-1288 were tested on these cell lines by using quantitative real-time polymerase chain reaction (qRT-PCR). An exogenous miR-1288 (mimic) was used to detect cell proliferation and cell cycle changes in SW480 using MTT calorimetric assay and flow cytometry, respectively. In addition, tissues from 122 patients with surgical resection of colorectum (82 adenocarcinomas, 20 ...
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    We aim to examine the miR-1288 expression in cancer cell lines and a large cohort of patients with colorectal cancer. Two colon cancer cell lines (SW480 and SW48) and one normal colonic epithelial cell line (FHC) were recruited. The miRNA expressions of miR-1288 were tested on these cell lines by using quantitative real-time polymerase chain reaction (qRT-PCR). An exogenous miR-1288 (mimic) was used to detect cell proliferation and cell cycle changes in SW480 using MTT calorimetric assay and flow cytometry, respectively. In addition, tissues from 122 patients with surgical resection of colorectum (82 adenocarcinomas, 20 adenomas, and 20 non-neoplastic tissues) were tested for miR-1288 expression by qRT-PCR. The colon cancer cell lines showed reduced expression of miR-1288 compared to normal colonic epithelial cell line. Over expression of miR-1288 in SW480 cell line showed increased cell proliferation and increased G2-M phase cells. In tissues, reduced miR-1288 expression was noted in majority of colorectal adenocarcinoma compared to colorectal adenoma and non-neoplastic tissues. Reduced or absent expression of miR-1288 was noted in 76% (n?=?62/82) of the cancers. The expression levels of miR-1288 were higher in distal colorectal adenocarcinomas (P?=?0.013) and in cancers of lower T staging (P?=?0.033). To conclude, alternation of miR-1288 expression is important in the progression of colorectal cancer. The differential regulation of miR-1288 was found to be related to cancer location and pathological staging in colorectal cancers.
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    Journal Title
    Molecular Carcinogenesis
    Volume
    53
    Issue
    s1
    DOI
    https://doi.org/10.1002/mc.21993
    Subject
    Oncology and carcinogenesis
    Biochemistry and cell biology
    Publication URI
    http://hdl.handle.net/10072/65098
    Collection
    • Journal articles

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