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dc.contributor.authorAlamein, Mohammad A
dc.contributor.authorWolvetang, Ernst J
dc.contributor.authorOvchinnikov, Dmitry A
dc.contributor.authorStephens, Sebastien
dc.contributor.authorSanders, Katherine
dc.contributor.authorWarnke, Patrick H
dc.date.accessioned2017-12-18T01:27:51Z
dc.date.available2017-12-18T01:27:51Z
dc.date.issued2015
dc.identifier.issn1932-6254
dc.identifier.doi10.1002/term.1960
dc.identifier.urihttp://hdl.handle.net/10072/65458
dc.description.abstractExpansion of pluripotent stem cells in defined media devoid of animal-derived feeder cells to generate multilayered three-dimensional (3D) bulk preparations or spheroids, rather than two-dimensional (2D) monolayers, is advantageous for many regenerative, biological or disease-modelling studies. Here we show that electrospun polymer matrices comprised of nanofibres that mimic the architecture of the natural fibrous extracellular matrix allow for feeder-free expansion of pluripotent human induced pluripotent stem cells (IPSCs) and human embryonic stem cells (HESCs) into multilayered 3D 'patty-like' spheroid structures in defined xeno-free culture medium. The observation that IPSCs and HESCs readily revert to 2D growth in the absence of the synthetic nanofibre membranes suggests that this 3D expansion behaviour is mediated by the physical microenvironment and artificial niche provided by the nanofibres only. Importantly, we could show that such 3D growth as patties maintained the pluripotency of cells as long as they were kept on nanofibres. The generation of complex multilayered 3D structures consisting of only pluripotent cells on biodegradable nanofibre matrices of the desired shape and size will enable both industrial-scale expansion and intricate organ–tissue engineering applications with human pluripotent stem cells, where simultaneous coupling of differentiation pathways of all germ layers from one stem cell source may be required for organ formation.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.publisherJohn Wiley & Sons
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1
dc.relation.ispartofpageto6
dc.relation.ispartofjournalJournal of Tissue Engineering and Regenerative Medicine
dc.rights.retentionY
dc.subject.fieldofresearchMedical and Health Sciences not elsewhere classified
dc.subject.fieldofresearchBiomedical Engineering
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchMedical Physiology
dc.subject.fieldofresearchcode119999
dc.subject.fieldofresearchcode0903
dc.subject.fieldofresearchcode1103
dc.subject.fieldofresearchcode1116
dc.titlePolymeric nanofibrous substrates stimulate Pluripotent Stem Cells to form three-dimensional multilayered patty-like spheroids in feeder-free culture and maintain their pluripotency
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codec1
dc.description.versionPost-print
gro.facultyGriffith Health Faculty
gro.description.notepublicThis publication has been entered into Griffith Research Online as an Advanced Online Version.
gro.rights.copyright© 2014 John Wiley & Sons, Ltd. This is the peer reviewed version of the following article: Polymeric nanofibrous substrates stimulate pluripotent stem cells to form three-dimensional multilayered patty-like spheroids in feeder-free culture and maintain their pluripotency, Journal of Tissue Engineering and Regenerative Medicine, pp. 1-6, 2014, which has been published in final form at 10.1002/term.1960. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html)
gro.hasfulltextFull Text
gro.griffith.authorStephens, Sebastien R.
gro.griffith.authorSanders, Katherine A.
gro.griffith.authorWarnke, Patrick H.
gro.griffith.authorAlamein, Mohammad


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