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  • High-throughput sequencing of plasma microRNA in chronic fatigue syndrome/myalgic encephalomyelitis

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    99294_1.pdf (586.4Kb)
    Author(s)
    Brenu, Ekua W
    Ashton, Kevin J
    Batovska, Jana
    Staines, Donald R
    Marshall-Gradisnik, Sonya M
    Griffith University Author(s)
    Staines, Donald R.
    Marshall-Gradisnik, Sonya M.
    Year published
    2014
    Metadata
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    Abstract
    Background MicroRNAs (miRNAs) are known to regulate many biological processes and their dysregulation has been associated with a variety of diseases including Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME). The recent discovery of stable and reproducible miRNA in plasma has raised the possibility that circulating miRNAs may serve as novel diagnostic markers. The objective of this study was to determine the role of plasma miRNA in CFS/ME. Results Using Illumina high-throughput sequencing we identified 19 miRNAs that were differentially expressed in the plasma of CFS/ME patients in comparison to non-fatigued ...
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    Background MicroRNAs (miRNAs) are known to regulate many biological processes and their dysregulation has been associated with a variety of diseases including Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME). The recent discovery of stable and reproducible miRNA in plasma has raised the possibility that circulating miRNAs may serve as novel diagnostic markers. The objective of this study was to determine the role of plasma miRNA in CFS/ME. Results Using Illumina high-throughput sequencing we identified 19 miRNAs that were differentially expressed in the plasma of CFS/ME patients in comparison to non-fatigued controls. Following RT-qPCR analysis, we were able to confirm the significant up-regulation of three miRNAs (hsa-miR-127-3p, hsa-miR-142-5p and hsa-miR-143-3p) in the CFS/ME patients. Conclusion Our study is the first to identify circulating miRNAs from CFS/ME patients and also to confirm three differentially expressed circulating miRNAs in CFS/ME patients, providing a basis for further study to find useful CFS/ME biomarkers.
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    Journal Title
    PlosOne
    Volume
    9
    Issue
    9
    DOI
    https://doi.org/10.1371/journal.pone.0102783
    Copyright Statement
    © 2014 Brenu et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License CCAL. (http://www.plos.org/journals/license.html)
    Subject
    Genomics
    Publication URI
    http://hdl.handle.net/10072/65528
    Collection
    • Journal articles

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