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  • Glycomic characterization of respiratory tract tissues of Ferrets: implications for its use in influenza virus infection studies

    Author(s)
    Jia, Nan
    Barclay, Wendy S
    Roberts, Kim
    Yen, Hui-Ling
    Chan, Renee WY
    Lam, Alfred KY
    Air, Gillian
    Peiris, JS Malik
    Dell, Anne
    Nicholls, John M
    Haslam, Stuart M
    Griffith University Author(s)
    Lam, Alfred K.
    Year published
    2014
    Metadata
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    Abstract
    The initial recognition between influenza virus and the host cell is mediated by interactions between the viral surface protein hemagglutinin and sialic acid-terminated glycoconjugates on the host cell surface. The sialic acid residues can be linked to the adjacent monosaccharide by a2-3- or a2-6-type glycosidic bonds. It is this linkage difference that primarily defines the species barrier of the influenza virus infection with a2-3 binding being associated with avian influenza viruses and a2-6 binding being associated with human strains. The ferret has been extensively used as an animal model to study the transmission of ...
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    The initial recognition between influenza virus and the host cell is mediated by interactions between the viral surface protein hemagglutinin and sialic acid-terminated glycoconjugates on the host cell surface. The sialic acid residues can be linked to the adjacent monosaccharide by a2-3- or a2-6-type glycosidic bonds. It is this linkage difference that primarily defines the species barrier of the influenza virus infection with a2-3 binding being associated with avian influenza viruses and a2-6 binding being associated with human strains. The ferret has been extensively used as an animal model to study the transmission of influenza. To better understand the validity of this model system, we undertook glycomic characterization of respiratory tissues of ferret, which allows a comparison of potential viral receptors to be made between humans and ferrets. To complement the structural analysis, lectin staining experiments were performed to characterize the regional distributions of glycans along the respiratory tract of ferrets. Finally, the binding between the glycans identified and the hemagglutinins of different strains of influenza viruses was assessed by glycan array experiments. Our data indicated that the respiratory tissues of ferret heterogeneously express both a2-3- and a2-6-linked sialic acids. However, the respiratory tissues of ferret also expressed the Sda epitope (NeuAca2-3(GalNAc߱-4)Gal߱-4GlcNAc) and sialylated N,N'-diacetyllactosamine (NeuAca2-6GalNAc߱-4GlcNAc), which have not been observed in the human respiratory tract surface epithelium. The presence of the Sda epitope reduces potential binding sites for avian viruses and thus may have implications for the usefulness of the ferret in the study of influenza virus infection.
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    Journal Title
    The Journal of Biological Chemistry
    Volume
    289
    Issue
    41
    DOI
    https://doi.org/10.1074/jbc.M114.588541
    Subject
    Medical and Health Sciences not elsewhere classified
    Chemical Sciences
    Biological Sciences
    Medical and Health Sciences
    Publication URI
    http://hdl.handle.net/10072/65544
    Collection
    • Journal articles

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