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dc.contributor.authorBakr, Mahmoud
dc.contributor.authorKelly, Wendy Lee
dc.contributor.authorBrunt, Athena Rachel
dc.contributor.authorDiessel, Gemma
dc.contributor.authorMassey, Ward
dc.contributor.authorMassa, Helen Maureen
dc.contributor.authorMorrison, Nigel Alexander
dc.contributor.authorForwood, Mark
dc.date.accessioned2014-12-10
dc.date.accessioned2015-01-13T03:45:43Z
dc.date.accessioned2017-03-02T00:00:51Z
dc.date.available2015-01-13T03:45:43Z
dc.date.available2017-03-02T00:00:51Z
dc.date.issued2014
dc.identifier.urihttp://hdl.handle.net/10072/65734
dc.description.abstractStress, or fatigue, fractures (Sfx), occur as a result of repetitive non-traumatic cyclic loading [1]. They are common in professional athletes, soldiers and dancers, and repair via a process of direct remodelling. Anti-inflammatory drugs (NSAIDs), commonly used in SFx patients, retard SFx healing, as do bisphosphonates (BPs)[1, 2]. Parathyroid hormone (PTH) has an anabolic effect that can accelerate bone remodelling and counteract effects of BP. Therefore, our aim was to investigate the short-term effect of a single PTH injection on the healing of SFx. Forty female wistar rats 300 g were allocated to PTH and vehicle (VEH) groups. 24 hours after Sfx, PTH group received a single dose of hPTH-(1-34) peptide (Sigma-Aldrich) (8 μg/100g) dissolved in 0.9% saline with 1% rat heat-inactivated serum. SFx was created in the right ulna of both groups using cyclic end-loading. We used the ulnar SFx model, allowing scrutiny of focal remodeling with a known time course and precise anatomical location. Both groups had an ulnar stress fracture induced in a single session. Ulnae of half of the groups were harvested two weeks after loading, the other half were harvested six weeks after loading. All ulnae were dissected, processed for histology and stained with Toluidine blue and TRAP for osteoclasts count. Histomorphometry was conducted using OsteomeasureTM. There were no differences between groups for cortical area, woven bone area or length of fracture. There was a trend for increased SFx porosity (resorption), erosion and area of new bone formation in PTH groups; but significantly increased osteoclast number when compared to the VEH group (P<0.01). These data provide evidence that a single PTH injection, 24 hours after SFx initiation, results in active changes in dynamics of bone remodelling that may accelerate healing. Additional data is now required to demonstrate the long-term effect on healing time, and potential for daily PTH injections on the healing of SFx.
dc.description.peerreviewedNo
dc.description.publicationstatusYes
dc.format.extent1185225 bytes
dc.format.mimetypeapplication/pdf
dc.publisher.urihttps://www.griffith.edu.au/
dc.relation.ispartofstudentpublicationY
dc.relation.ispartofconferencenameGold Coast Health and Medical Research Conference
dc.relation.ispartofconferencetitleGold Coast Health and Medical Research Conference
dc.relation.ispartofdatefrom2014-12-04
dc.relation.ispartofdateto2014-12-05
dc.relation.ispartoflocationQueensland, Australia
dc.rights.retentionY
dc.subject.fieldofresearchCell Physiology
dc.subject.fieldofresearchcode111601
dc.titleThe effect of a single Parathyroid Hormone (PTH) injection on the healing of stress fractures.
dc.typeConference output
dc.type.descriptionE3 - Conferences (Extract Paper)
dc.type.codee3
gro.facultyGriffith Health Faculty
gro.rights.copyright© The Author(s) 2014.The attached file is posted here with permission of the copyright owners for your personal use only. No further distribution permitted. For information about this conference please refer to the publisher's website or contact the authors.
gro.hasfulltextFull Text
gro.griffith.authorMorrison, Nigel A.
gro.griffith.authorForwood, Mark R.
gro.griffith.authorMassa, Helen M.
gro.griffith.authorBrunt, Athena R.
gro.griffith.authorKelly, Wendy L.
gro.griffith.authorMassey, Ward
gro.griffith.authorDiessel, Gemma
gro.griffith.authorBakr, Mahmoud


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