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dc.contributor.authorLochner, A
dc.contributor.authorMarais, E
dc.contributor.authorDu Toit, Eugene
dc.contributor.authorMoolman, J
dc.date.accessioned2009-06-29
dc.date.accessioned2017-03-02T00:33:49Z
dc.date.available2015-02-10T04:40:31Z
dc.date.available2017-03-02T00:33:49Z
dc.date.issued2002
dc.identifier.issn0077-8923
dc.identifier.doi10.1111/j.1749-6632.2002.tb04084.x
dc.identifier.urihttp://hdl.handle.net/10072/66101
dc.description.abstractThe role of NO in the classic ischemic preconditioning phenomenon of the myocardium is not well defined, and was investigated by using the isolated perfused rat heart as a model. Hearts were preconditioned with 3 × 5 minute ischemia in the presence and absence of the NOS inhibitors l-NAME (50 μM) and l-NNA (50 μM), and the guanylyl cyclase inhibitor ODQ (20 μM). These inhibitors significantly attenuated the protective effect of preconditioning against 25-min global ischemia (as measured by functional recovery), specifically if administered during the triggering phase. Cyclic infusions (3 × 5 min) of the NO-donors SNAP (50 μM) and SNP (100 μM) elicited protection against both 25-min global or low-flow ischemia. Hearts preconditioned with NO donors displayed significantly superior functional reserve, if stimulated with adrenaline, compared to hearts preconditioned with ischemia. Although the NO donors SNAP and SNP both activated p38 MAPK during the preconditioning protocol, protection was accompanied by significantly decreased p38 MAPK activity during sustained ischemia, as was the case in ischemic preconditioning. We conclude that (1) NO is a trigger for classic preconditioning, (2) cGMP generation plays an important role in its protection, (3) attenuation of p38 MAPK during sustained ischemia accompanies NO preconditioning and may mediate cardiac protection, and (4) preconditioning with NO may be more advantageous than using ischemia.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.publisher.placeOxeen, England
dc.relation.ispartofpagefrom402
dc.relation.ispartofpageto414
dc.relation.ispartofjournalAnnals of the New York Acadamy of Science
dc.relation.ispartofvolume962
dc.subject.fieldofresearchCardiology (incl. Cardiovascular Diseases)
dc.subject.fieldofresearchcode110201
dc.titleNitric Oxide Triggers Classic Ischemic Preconditioning
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codec1x
gro.facultyGriffith Health Faculty
gro.date.issued2015-02-10T04:40:31Z
gro.hasfulltextNo Full Text
gro.griffith.authorDu Toit, Eugene


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