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  • Laboratory validation of enzyme-immunoassays for the non-invasive quantification of reproductive and stress hormone in amphibians

    Author(s)
    Narayan, Edward Jitik
    Griffith University Author(s)
    Narayan, Edward J.
    Year published
    2014
    Metadata
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    Abstract
    Quantification of reproductive and stress hormones entirely non-invasively using amphibian urine samples has provided invaluable tool for understanding the biological functioning of reproductive and stress endocrine axis in rare, endangered and threatened species. This technical paper, I describe step by step methods for the laboratory validation of enzyme-immunoassays (EIAs) for the quantification of reproductive hormones (testosterone, oestradiol and progesterone) and stress hormone (corticosterone) entirely non-invasively in amphibian urine samples. These laboratory validation steps include (1) Accuracy/recovery checks, ...
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    Quantification of reproductive and stress hormones entirely non-invasively using amphibian urine samples has provided invaluable tool for understanding the biological functioning of reproductive and stress endocrine axis in rare, endangered and threatened species. This technical paper, I describe step by step methods for the laboratory validation of enzyme-immunoassays (EIAs) for the quantification of reproductive hormones (testosterone, oestradiol and progesterone) and stress hormone (corticosterone) entirely non-invasively in amphibian urine samples. These laboratory validation steps include (1) Accuracy/recovery checks, (2) Parallelism and (3) Quality control. I also present standard assay methods and together these will enable the advancement of non-invasive reproductive and stress endocrinology of amphibians.
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    Journal Title
    Protocol Exchange
    Volume
    n/a
    DOI
    https://doi.org/10.1038/protex.2014.009
    Subject
    Analytical Biochemistry
    Comparative Physiology
    Publication URI
    http://hdl.handle.net/10072/66352
    Collection
    • Journal articles

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