Estrogen receptor alpha gene expression in breast cancer tissues from the Iranian population - a pilot study

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Author(s)
Hosseini, Arezoo
Gopalan, Vinod
Nassiri, Mohammadreza
Ghaffarzadehgan, Kamran
Aslaminejad, Ali
Ghovvati, Shahrokh
Smith, Robert A
Lam, Alfred KY
Year published
2014
Metadata
Show full item recordAbstract
Estrogen receptor alpha (ERa) is one of the major sub-types of estrogen receptors. ERa plays an important role in cellular proliferation and differentiation, chiefly in mammary tissues. In the present study we aimed to quantify of ERa mRNA and protein expression in breast tissues from the Iranian population using a real-time PCR assay. Twenty nine breast tissues including 19 adenocarcinomas and 10 normal controls were recruited from the Iranian population. mRNA extraction and cDNA synthesis were performed from these tissues using commercial kits. ERa mRNA and protein expression was quantified using real-time PCR and ...
View more >Estrogen receptor alpha (ERa) is one of the major sub-types of estrogen receptors. ERa plays an important role in cellular proliferation and differentiation, chiefly in mammary tissues. In the present study we aimed to quantify of ERa mRNA and protein expression in breast tissues from the Iranian population using a real-time PCR assay. Twenty nine breast tissues including 19 adenocarcinomas and 10 normal controls were recruited from the Iranian population. mRNA extraction and cDNA synthesis were performed from these tissues using commercial kits. ERa mRNA and protein expression was quantified using real-time PCR and immunohistochemistry respectively. The results showed high expression of ERa mRNA (68%) and protein (53%) in the majority of breast cancer tissues compared to normal breast tissues (p= 0.035). Also, high ERa mRNA was associated with tumour size of breast carcinomas. In this study, we first reported the expression of ERa in Iranian patients with breast cancers and demonstrated prevalence of the expression to be similar to breast cancers noted in other populations.
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View more >Estrogen receptor alpha (ERa) is one of the major sub-types of estrogen receptors. ERa plays an important role in cellular proliferation and differentiation, chiefly in mammary tissues. In the present study we aimed to quantify of ERa mRNA and protein expression in breast tissues from the Iranian population using a real-time PCR assay. Twenty nine breast tissues including 19 adenocarcinomas and 10 normal controls were recruited from the Iranian population. mRNA extraction and cDNA synthesis were performed from these tissues using commercial kits. ERa mRNA and protein expression was quantified using real-time PCR and immunohistochemistry respectively. The results showed high expression of ERa mRNA (68%) and protein (53%) in the majority of breast cancer tissues compared to normal breast tissues (p= 0.035). Also, high ERa mRNA was associated with tumour size of breast carcinomas. In this study, we first reported the expression of ERa in Iranian patients with breast cancers and demonstrated prevalence of the expression to be similar to breast cancers noted in other populations.
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Journal Title
Asian Pacific Journal of Cancer Prevention
Volume
15
Issue
20
Copyright Statement
© The Author(s) 2014. The attached file is reproduced here in accordance with the copyright policy of the publisher. For information about this journal please refer to the journal’s website or contact the authors.
Subject
Clinical sciences
Oncology and carcinogenesis