Continuous biotransformation of bisphenol A and diclofenac by laccase in an enzymatic membrane reactor
Author(s)
Nguyen, Luong N
Hai, Faisal I
Price, William E
Leusch, Frederic DL
Roddick, Felicity
McAdam, Ewan J
Magram, Saleh F
Nghiem, Long D
Griffith University Author(s)
Year published
2014
Metadata
Show full item recordAbstract
A novel enzymatic membrane reactor (EMR) was explored for continuous removal of two trace organic contaminants (TrOCs) ubiquitously detected in wastewater - namely bisphenol A (BPA) and diclofenac (DCF) - by a commercially available laccase from Aspergillus oryzae. An ultrafiltration membrane prevented washout of the enzyme and allowed continuous removal of BPA and DCF (>85% and >60%, respectively) under a loading rate of 570 ᠷ0 姯L d (BPA) and 480 ᠴ0 姯L d (DCF). The BPA and DCF removal could be further improved to >95% and >80%, respectively, by dosing to the EMR a natural redox-mediator compound - syringaldehyde (5 卩 - ...
View more >A novel enzymatic membrane reactor (EMR) was explored for continuous removal of two trace organic contaminants (TrOCs) ubiquitously detected in wastewater - namely bisphenol A (BPA) and diclofenac (DCF) - by a commercially available laccase from Aspergillus oryzae. An ultrafiltration membrane prevented washout of the enzyme and allowed continuous removal of BPA and DCF (>85% and >60%, respectively) under a loading rate of 570 ᠷ0 姯L d (BPA) and 480 ᠴ0 姯L d (DCF). The BPA and DCF removal could be further improved to >95% and >80%, respectively, by dosing to the EMR a natural redox-mediator compound - syringaldehyde (5 卩 - believed to act as an electron shuttle between laccase and the target pollutants. Of particular interest was the significant retention of the TrOCs on the gel layer of enzyme on the membrane surface, and their subsequent biodegradation.
View less >
View more >A novel enzymatic membrane reactor (EMR) was explored for continuous removal of two trace organic contaminants (TrOCs) ubiquitously detected in wastewater - namely bisphenol A (BPA) and diclofenac (DCF) - by a commercially available laccase from Aspergillus oryzae. An ultrafiltration membrane prevented washout of the enzyme and allowed continuous removal of BPA and DCF (>85% and >60%, respectively) under a loading rate of 570 ᠷ0 姯L d (BPA) and 480 ᠴ0 姯L d (DCF). The BPA and DCF removal could be further improved to >95% and >80%, respectively, by dosing to the EMR a natural redox-mediator compound - syringaldehyde (5 卩 - believed to act as an electron shuttle between laccase and the target pollutants. Of particular interest was the significant retention of the TrOCs on the gel layer of enzyme on the membrane surface, and their subsequent biodegradation.
View less >
Journal Title
International Biodeterioration & Biodegradation
Volume
95
Issue
Part A
Subject
Environmental sciences
Environmental management
Biological sciences
Analytical biochemistry