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  • A novel pathogenesis-related protein (LcPR4a) from lentil, and its involvement in defence against Ascochyta lentis

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    Author(s)
    Vaghefi, Niloofar
    Mustafa, Barkat M
    Dulal, Nabeen
    Selby-Pham, Jamie
    Taylor, Paul WJ
    Ford, Rebecca
    Griffith University Author(s)
    Ford, Rebecca
    Year published
    2013
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    Abstract
    A novel pathogenesis-related protein 4 (PR4) encoding gene, LcPR4a, was induced in Lens culinaris following Ascochyta lentis infection. LcPR4a encodes a predicted 146 amino acid protein of 15.8 kDa. The putative LcPR4a protein belongs to the class II PR4 family and has close phylogenetic affinity to PR4 proteins from related species. qPCR analysis revealed differential expression of the LcPR4a gene upon Ascochyta lentis infection in both resistant and susceptible cultivars. This, combined with preliminary in vitro antifungal assays of the recombinant protein expressed in E. coli, suggests the potential important role of ...
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    A novel pathogenesis-related protein 4 (PR4) encoding gene, LcPR4a, was induced in Lens culinaris following Ascochyta lentis infection. LcPR4a encodes a predicted 146 amino acid protein of 15.8 kDa. The putative LcPR4a protein belongs to the class II PR4 family and has close phylogenetic affinity to PR4 proteins from related species. qPCR analysis revealed differential expression of the LcPR4a gene upon Ascochyta lentis infection in both resistant and susceptible cultivars. This, combined with preliminary in vitro antifungal assays of the recombinant protein expressed in E. coli, suggests the potential important role of LcPR4a in the defence response of lentil to Ascochyta lentis attack.
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    Journal Title
    Phytopathologia Mediterranea
    Volume
    52
    Issue
    1
    Publisher URI
    https://oajournals.fupress.net/index.php/pm/article/view/5688
    Copyright Statement
    © 2013 Firenze University Press. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
    Subject
    Gene expression (incl. microarray and other genome-wide approaches)
    Horticultural production
    Publication URI
    http://hdl.handle.net/10072/67310
    Collection
    • Journal articles

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