dc.contributor.author | Islam, Tasneem | |
dc.contributor.author | Butler, Melissa | |
dc.contributor.author | Sikkander, Sulthan A. | |
dc.contributor.author | Toida, Toshihiko | |
dc.contributor.author | Linhardt, Robert J. | |
dc.date.accessioned | 2006-08-06 | |
dc.date.accessioned | 2015-04-29T23:47:00Z | |
dc.date.accessioned | 2017-03-02T01:08:37Z | |
dc.date.available | 2015-04-29T23:47:00Z | |
dc.date.available | 2017-03-02T01:08:37Z | |
dc.date.issued | 2002 | |
dc.identifier.issn | 0008-6215 | |
dc.identifier.doi | 10.1016/S0008-6215(02)00229-X | |
dc.identifier.uri | http://hdl.handle.net/10072/67448 | |
dc.description.abstract | Porcine mucosal heparin was fragmented into low-mol.-wt. (LMW) heparin by treatment of periodate-oxidized heparin with sodium hydroxide, followed by redn. with sodium borohydride and acid hydrolysis. Gradient polyacrylamide gel electrophoresis anal. showed a mixt. of heparin fragments with an av. size of eight disaccharide units. 1D 1H NMR showed two-thirds of the N-acetyl groups were lost on periodate cleavage, suggesting cleavage had occurred at the glucopyranosyluronic acid (GlcpA) and idopyranosyluronic acid (IdopA) residues located within and adjacent to the antithrombin III (ATIII) binding site. The N-acetyl glucopyranose (GlcpNAc) residue was lost on workup. The GlcpA residue, within the ATIII binding site, is on the non-reducing side of the N-sulfo,3,6-O-sulfo glycopyranosylamine (GlcpNS3S6S) residue. Thus, periodate cleaved heparin should be enriched in GlcpNS3S6S residues. Two-dimensional correlation spectroscopy (2D COSY) confirmed that LMW heparin prepd. through periodate cleavage contained GlcpNS3S6S at its non-reducing end. As expected, this LMW heparin also showed reduced ATIII mediated anti-factor IIa and anti-factor Xa activities. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.publisher | Elsevier Science | |
dc.publisher.place | U.S.A | |
dc.relation.ispartofpagefrom | 2239 | |
dc.relation.ispartofpageto | 2243 | |
dc.relation.ispartofissue | 21-23 | |
dc.relation.ispartofjournal | Carbohydrate Research | |
dc.relation.ispartofvolume | 337 | |
dc.subject.fieldofresearch | Medicinal and Biomolecular Chemistry | |
dc.subject.fieldofresearch | Organic Chemistry | |
dc.subject.fieldofresearch | Biochemistry and Cell Biology | |
dc.subject.fieldofresearchcode | 0304 | |
dc.subject.fieldofresearchcode | 0305 | |
dc.subject.fieldofresearchcode | 0601 | |
dc.title | Further evidence that periodate cleavage of heparin occurs primarily through the antithrombin binding site | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | c1x | |
gro.faculty | Institute for Glycomics | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Islam, Tasneem | |