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dc.contributor.authorTerman, A
dc.contributor.authorNeuzil, J
dc.contributor.authorKagedal, K
dc.contributor.authorOllinger, K
dc.contributor.authorBrunk, UT
dc.contributor.editorU. Lendahl
dc.date.accessioned2017-05-03T16:35:09Z
dc.date.available2017-05-03T16:35:09Z
dc.date.issued2002
dc.date.modified2009-09-08T08:02:01Z
dc.identifier.issn0014-4827
dc.identifier.doi10.1006/excr.2001.5441
dc.identifier.urihttp://hdl.handle.net/10072/6783
dc.description.abstractTo better understand the role of lysosomes in apoptosis, we compared the responses to apoptotic stimuli of normal fibroblasts with those of inclusion cells (I-cells), i.e., fibroblasts with impaired function of lysosomal enzymes due to their missorting and ensuing nonlysosomal localization. Although both cell types did undergo apoptosis when exposed to the lysosomotropic detergent MSDH, the redox-cycling quinone naphthazarin, or the protein kinase inhibitor staurosporine, I-cells exerted a markedly decreased response to these agonists than did normal fibroblasts. Furthermore, leupeptin and pepstatin A (inhibitors of cysteine and aspartic proteases, respectively) suppressed staurosporine-induced apoptosis of normal fibroblasts, whereas survival of I-cells was unaffected. These findings give further support for the involvement of lysosomal enzymes in apoptosis and suggest I-cells as a suitable model for studying the role of lysosomes in programmed cell death.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier Science
dc.publisher.placeUnited States
dc.publisher.urihttp://www.elsevier.com/wps/find/journaldescription.cws_home/622826/description#description
dc.relation.ispartofpagefrom9
dc.relation.ispartofpageto15
dc.relation.ispartofissue1
dc.relation.ispartofjournalExperimental Cell Research
dc.relation.ispartofvolume274
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3202
dc.titleDecreased apoptotic response of inclusion-cell disease fibroblasts: a consequence of lysosomal enzyme missorting?
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.rights.copyright© 2002 Elsevier : Reproduced in accordance with the copyright policy of the publisher : This journal is available online - use hypertext links.
gro.date.issued2002
gro.hasfulltextNo Full Text
gro.griffith.authorNeuzil, Jiri


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