Cytokine profiles of chronic fatigue syndrome and multiple sclerosis patients.
Author(s)
Wong, Naomi
Brenu, Ekua
Hardcastle, Sharni
Johnston, Samantha
Thao, Nguyen
Huth, Teilah
Hawthorn, Ally
Passmore, Rachel
Ramos, Sandra
Salajegheh, Ali
Broadley, Simon
Staines, Don
Marshall-Gradisnik, Sonya
Griffith University Author(s)
Year published
2014
Metadata
Show full item recordAbstract
Aims
Chronic Fatigue Syndrome (CFS) and Multiple Sclerosis (MS) are both disorders with severe neuroimmune symptoms, including cognitive impairment, immune dysfunction and abnormal cytokine expression. The purpose of this study was to assess the T helper (Th) 1, Th2 and Th17 cytokine profiles of CFS and MS patients.
Methods
This study measured the cytokine profiles of CFS patients (n = 16; mean age 49.88, SD 9.542), MS patients (n = 16; mean age 52.75, SD 12.809) and healthy controls (n = 16; mean age 50.06, SD 11.846). The diagnostic selection method used to identify CFS patients was the International Consensus Criteria. ...
View more >Aims Chronic Fatigue Syndrome (CFS) and Multiple Sclerosis (MS) are both disorders with severe neuroimmune symptoms, including cognitive impairment, immune dysfunction and abnormal cytokine expression. The purpose of this study was to assess the T helper (Th) 1, Th2 and Th17 cytokine profiles of CFS and MS patients. Methods This study measured the cytokine profiles of CFS patients (n = 16; mean age 49.88, SD 9.542), MS patients (n = 16; mean age 52.75, SD 12.809) and healthy controls (n = 16; mean age 50.06, SD 11.846). The diagnostic selection method used to identify CFS patients was the International Consensus Criteria. Cytokines were measured from serum using a Bio-Plex Pro™ kit for Th1 (IFN-γ, TNF-α), Th2 (IL-4, IL-6, IL-10, IL-13) and Th17 (IL-17) cell cytokines. Results When the three groups were compared, it was found that TNF-α (p = 0.011, p = 0.012), IL-4 (p = 0.000, p = 0.000), IL-6 (p = 0.039, p = 0.031), IL-13 (p = 0.000, p = 0.000) and IL-17 (p = 0.004, p = 0.038) were all significantly higher in MS patients compared with CFS patients and controls respectively. However, in the MS patients and CFS patients, serum levels of IL-13 and IFN-γ were significantly higher (p ⩽ 0.001) compared with the controls. IFN-γ was significantly different between MS and CFS (p = 0.001), with MS exhibiting higher IFN-γ serum levels. Conclusion Cytokines patterns supported both Th1 and Th2 cytokine profiles in CFS and MS. Similarities in the cytokine profiles of MS and CFS, combined with the already acknowledged similarities in immune cell function and symptoms, suggests a neuroimmune pathology for CFS with parallels to that of MS. Additional studies into the cytokine profiles of both CFS and MS should be conducted, with larger sample sizes, to further expand the understanding of the pathologies of both disorders.
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View more >Aims Chronic Fatigue Syndrome (CFS) and Multiple Sclerosis (MS) are both disorders with severe neuroimmune symptoms, including cognitive impairment, immune dysfunction and abnormal cytokine expression. The purpose of this study was to assess the T helper (Th) 1, Th2 and Th17 cytokine profiles of CFS and MS patients. Methods This study measured the cytokine profiles of CFS patients (n = 16; mean age 49.88, SD 9.542), MS patients (n = 16; mean age 52.75, SD 12.809) and healthy controls (n = 16; mean age 50.06, SD 11.846). The diagnostic selection method used to identify CFS patients was the International Consensus Criteria. Cytokines were measured from serum using a Bio-Plex Pro™ kit for Th1 (IFN-γ, TNF-α), Th2 (IL-4, IL-6, IL-10, IL-13) and Th17 (IL-17) cell cytokines. Results When the three groups were compared, it was found that TNF-α (p = 0.011, p = 0.012), IL-4 (p = 0.000, p = 0.000), IL-6 (p = 0.039, p = 0.031), IL-13 (p = 0.000, p = 0.000) and IL-17 (p = 0.004, p = 0.038) were all significantly higher in MS patients compared with CFS patients and controls respectively. However, in the MS patients and CFS patients, serum levels of IL-13 and IFN-γ were significantly higher (p ⩽ 0.001) compared with the controls. IFN-γ was significantly different between MS and CFS (p = 0.001), with MS exhibiting higher IFN-γ serum levels. Conclusion Cytokines patterns supported both Th1 and Th2 cytokine profiles in CFS and MS. Similarities in the cytokine profiles of MS and CFS, combined with the already acknowledged similarities in immune cell function and symptoms, suggests a neuroimmune pathology for CFS with parallels to that of MS. Additional studies into the cytokine profiles of both CFS and MS should be conducted, with larger sample sizes, to further expand the understanding of the pathologies of both disorders.
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Conference Title
CYTOKINE
Volume
70
Issue
1
Publisher URI
Subject
Biochemistry and cell biology
Genetics
Immunology