Comprehensive Analysis of Peripheral B Cell Phenotypes in Chronic Fatigue Syndrome
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Author(s)
Fuller, Kirsty
Brenu, Ekua
Huth, Teilah Kathryn
Hardcastle, Sharni Lee
Johnston, Samantha
Nguyen, Thao
Staines, Don
Marshall-Gradisnik, Sonya
Griffith University Author(s)
Year published
2014
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Objectives: B cells are key adaptive immune cells which are subdivided into phenotypes, each of which has a specialised effector immune function. Perturbations in the levels of one or more of the subtypes may lead to immune dysregulation. Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME) is a multifactorial illness which has an unknown cause and no current clinical biomarker. Immune dysregulation has come to be associated with CFS/ME however the precise role that B cells play in the illness pathogenesis remains unclear. Hence, this study aims to further elucidate the role of B cells in CFS/ME via the comprehensive ...
View more >Objectives: B cells are key adaptive immune cells which are subdivided into phenotypes, each of which has a specialised effector immune function. Perturbations in the levels of one or more of the subtypes may lead to immune dysregulation. Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME) is a multifactorial illness which has an unknown cause and no current clinical biomarker. Immune dysregulation has come to be associated with CFS/ME however the precise role that B cells play in the illness pathogenesis remains unclear. Hence, this study aims to further elucidate the role of B cells in CFS/ME via the comprehensive analysis of multiple peripheral B cells phenotypes. Methods: Peripheral blood samples were collected from CFS/ME patients and non-fatigued healthy controls (HC). Cells were then incubated with the appropriate B cell antibody panels, prior to red blood cell lysing. Cells were analysed via flow cytometry, with the lymphocyte gate specific for CD19+ B cells. Results: Differential levels of multiple B cell subsets were observed in CFS/ME patients when compared to HCs. Conclusion: Results from the current study further confirm a potential involvement of B cells in CFS/ME. Thus, further studies should be conducted to confirm whether these observed alterations contribute to the symptom profile that is characteristic of CFS/ME.
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View more >Objectives: B cells are key adaptive immune cells which are subdivided into phenotypes, each of which has a specialised effector immune function. Perturbations in the levels of one or more of the subtypes may lead to immune dysregulation. Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME) is a multifactorial illness which has an unknown cause and no current clinical biomarker. Immune dysregulation has come to be associated with CFS/ME however the precise role that B cells play in the illness pathogenesis remains unclear. Hence, this study aims to further elucidate the role of B cells in CFS/ME via the comprehensive analysis of multiple peripheral B cells phenotypes. Methods: Peripheral blood samples were collected from CFS/ME patients and non-fatigued healthy controls (HC). Cells were then incubated with the appropriate B cell antibody panels, prior to red blood cell lysing. Cells were analysed via flow cytometry, with the lymphocyte gate specific for CD19+ B cells. Results: Differential levels of multiple B cell subsets were observed in CFS/ME patients when compared to HCs. Conclusion: Results from the current study further confirm a potential involvement of B cells in CFS/ME. Thus, further studies should be conducted to confirm whether these observed alterations contribute to the symptom profile that is characteristic of CFS/ME.
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Conference Title
Comprehensive Analysis of Peripheral B Cell Phenotypes in Chronic Fatigue Syndrome
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Copyright Statement
© 2014 International Congress on Autoimmunity. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the conference's website for access to the definitive, published version.
Subject
Cellular Immunology