dc.contributor.author | Hardcastle, Sharni | |
dc.contributor.author | Brenu, Ekua | |
dc.contributor.author | Johnston, Samantha | |
dc.contributor.author | Nguyen, Thao | |
dc.contributor.author | Huth, Teilah | |
dc.contributor.author | Baljit Singh, Manprit Kaur | |
dc.contributor.author | Ramos, Sandra | |
dc.contributor.author | Ariana, Armin | |
dc.contributor.author | Staines, Don | |
dc.contributor.author | Marshall-Gradisnik, Sonya | |
dc.date.accessioned | 2017-05-03T15:39:09Z | |
dc.date.available | 2017-05-03T15:39:09Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 2155-9899 | |
dc.identifier.doi | 10.4172/2155-9899.1000190 | |
dc.identifier.uri | http://hdl.handle.net/10072/68881 | |
dc.description.abstract | Objective: Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME) is a disabling illness, characterised by persistent, debilitating fatigue and a multitude of symptoms. Immunological alterations are prominent in CFS/ME cases, however little is known about the relationship between CFS/ME severity and the extent of immunological dysfunction. The purpose of this study was to assess innate and adaptive immune cell phenotypes and function of two groups of CFS/ME patients, bedridden (severe) and mobile (moderate). Methods: CFS/ME participants were defined using the Centres for Disease Prevention and Control (1994 CDC) Criteria for CFS/ME. Participants were grouped into healthy controls (n=22, age=40.14 ᠲ.38), moderate/ mobile (n=23; age=42.52 ᠲ.63) and severe/bedridden (n=18; age=39.56 ᠱ.51) CFS/ME patients. Flow cytometric protocols were used to examine neutrophil, monocyte, dendritic cells (DCs), iNKT, Treg, B, ?d and CD8+ T cell phenotypes, NK cytotoxic activity and receptors. Results: The present data found that CFS/ME patients demonstrated significant decreases in NK cytotoxic activity, transitional and regulatory B cells, ?d1T cells, KIR2DL1/DS1, CD94+ and KIR2DL2/L3. Significant increases in CD56- CD16+NKs, CD56dimCD16- and CD56brightCD16-/dim NKs, DCs, iNKT phenotypes, memory and naive B cells were also shown in CFS/ME participants. Severe CFS/ME patients demonstrated increased CD14- CD16+ DCs, memory and naﶥ B cells, total iNKT, iNKT cell and NK phenotypes compared to moderate CFS/ME patients. Conclusion: This study is the first to determine alterations in NK, iNKT, B, DC and ?d T cell phenotypes in both moderate and severe CFS/ME patients. Immunological alterations are present in innate and adaptive immune cells and sometimes, immune deregulation appears worse in CFS/ME patients with more severe symptoms. It may be appropriate for CFS/ME patient severity subgroups to be distinguished in both clinical and research settings to extricate further immunological pathologies that may not have been previously reported. | |
dc.description.peerreviewed | Yes | |
dc.description.publicationstatus | Yes | |
dc.format.extent | 1029868 bytes | |
dc.format.mimetype | application/pdf | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Omics Publishing Group | |
dc.publisher.place | United States | |
dc.relation.ispartofstudentpublication | Y | |
dc.relation.ispartofpagefrom | 1000190-1 | |
dc.relation.ispartofpageto | 1000190-9 | |
dc.relation.ispartofissue | 1 | |
dc.relation.ispartofjournal | Journal of Clinical & Cellular Immunology | |
dc.relation.ispartofvolume | 5 | |
dc.rights.retention | Y | |
dc.subject.fieldofresearch | Cellular Immunology | |
dc.subject.fieldofresearchcode | 110704 | |
dc.title | Analysis of the Relationship between Immune Dysfunction and Symptom Severity in Patients with Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME) | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.rights.copyright | © 2014 Hardcastle SL, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Staines, Don R. | |
gro.griffith.author | Ariana, Armin S. | |
gro.griffith.author | Hardcastle, Sharni L. | |
gro.griffith.author | Huth, Teilah K. | |
gro.griffith.author | Ramos, Sandra B. | |
gro.griffith.author | Baljit Singh, Manprit Kaur K. | |
gro.griffith.author | Marshall-Gradisnik, Sonya M. | |
gro.griffith.author | Johnston, Samantha | |
gro.griffith.author | Brenu, Ekua | |
gro.griffith.author | Nguyen, Thao | |