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dc.contributor.authorBrenu, Ekua
dc.contributor.authorStaines, Don
dc.contributor.authorMarshall-Gradisnik, Sonya
dc.date.accessioned2017-05-03T16:10:59Z
dc.date.available2017-05-03T16:10:59Z
dc.date.issued2014
dc.identifier.issn2155-9899
dc.identifier.doi10.4172/2155-9899.1000228
dc.identifier.urihttp://hdl.handle.net/10072/68935
dc.description.abstractObjective: Methylation is known to regulate biological processes and alterations in methylation patterns have been associated with a variety of diseases. Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME) is an unexplained disorder associated with immunological and molecular changes. CD4+T cells specifically, regulatory T cells (Tregs) have been implicated in CFS/ME patients where significant increases in Tregs have been observed in these patients. Therefore the objective of this study was to examine methylation in CD4+T cells from CFS/ME patients. Methods: The study comprised twenty-five CFS/ME participants and eighteen controls aged between 25-60 years. A volume of 20 ml whole blood was collected from each participant and peripheral blood mononuclear cells were isolated via density gradient centrifugation. A negative isolation kit was used to isolate the CD4+T cells from the peripheral blood samples. Genome wide methylation studies were performed on isolated CD4+T cells using the Illumina Infinium 450 K Human methylation array system. Data analysis was performed using Genome studio and Partek Enrichment software. Results: 120 CpGs were observed to be differentially methylated in the CFS/ME patients in comparison to the controls. Of these 70 were associated with known genes. The majority of the differential methylated regions in the CFS/ME patients were hypomethylated. Additionally, most of the genes with differentially methylated regions in the CFS/ME patients were responsible for apoptosis, cell development, cell function and metabolic activity. Conclusion: The present study demonstrates that epigenetic changes in CD4+T cells may have a potential role in the immunological changes observed in CFS/ME patients.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.format.extent726545 bytes
dc.format.mimetypeapplication/pdf
dc.languageEnglish
dc.language.isoeng
dc.publisherOmics Publishing Group
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom228-1
dc.relation.ispartofpageto228-14
dc.relation.ispartofissue3
dc.relation.ispartofjournalJournal of Clinical & Cellular Immunology
dc.relation.ispartofvolume5
dc.rights.retentionY
dc.subject.fieldofresearchCellular Immunology
dc.subject.fieldofresearchcode110704
dc.titleMethylation Profile of CD4+ T Cells in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Medical Science
gro.rights.copyright© 2014 Brenu EW, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
gro.hasfulltextFull Text
gro.griffith.authorStaines, Don R.
gro.griffith.authorMarshall-Gradisnik, Sonya M.
gro.griffith.authorBrenu, Ekua


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