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dc.contributor.authorUtama, B
dc.contributor.authorKennedy, D
dc.contributor.authorRu, KL
dc.contributor.authorMattick, JS
dc.date.accessioned2017-05-03T11:12:26Z
dc.date.available2017-05-03T11:12:26Z
dc.date.issued2002
dc.date.modified2007-03-14T21:39:30Z
dc.identifier.issn1356-9597
dc.identifier.doi10.1046/j.1356-9597.2001.00507.x
dc.identifier.urihttp://hdl.handle.net/10072/7015
dc.description.abstractBackground: The nucleolus is the site of rRNA synthesis and processing in eukaryotic cells, but its composition remains poorly understood. Results: We have identified a novel nucleolar RNA-associated protein (Nrap) which is highly conserved from yeast (Saccharomyces cerevisiae) to human, with homologues in mouse, Drosophila melanogaster, Caenorhabditis elegans, Arabidopsis thaliana, Schizosaccharomyces pombe, and other species. In the mouse, we have found that Nrap is ubiquitously expressed and is specifically localized in the nucleolus. We have also identified splice variants in human and mouse, and defined the intron-exon structure of the human Nrap gene. Nrap is inherited into daughter nuclei by associating with the condensed chromosomes during mitosis. RNase treatment of permeabilized cells indicated that the nucleolar localization of Nrap is RNA dependent. The effects of actinomycin D, cycloheximide and 5,6-dichloro-߭d-ribofuranosyl-benzimidazole on Nrap expression and distribution in cultured cells suggest that Nrap is associated with the pre-rRNA transcript. Conclusions: Nrap is a large nucleolar protein (of more than 1000 amino acids), and is a new class of protein with new structural and functional motifs. Nrap appears to be associated with ribosome biogenesis by interacting with pre-rRNA primary transcript.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherBlackwell Synergy
dc.publisher.placeUSA
dc.publisher.urihttp://www.blackwell-synergy.com/doi/full/10.1046/j.1356-9597.2001.00507.x
dc.relation.ispartofpagefrom115
dc.relation.ispartofpageto132
dc.relation.ispartofjournalGenes to Cells
dc.relation.ispartofvolume7
dc.subject.fieldofresearchBiochemistry and cell biology
dc.subject.fieldofresearchGenetics
dc.subject.fieldofresearchcode3101
dc.subject.fieldofresearchcode3105
dc.titleIsolation and characterization of a new nucleolar protein, Nrap, that is conserved from yeast to humans
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Sciences, School of Natural Sciences
gro.rights.copyright© 2002 Blackwell Publishing. The definitive version is available at [www.blackwell-synergy.com.]
gro.date.issued2002
gro.hasfulltextNo Full Text
gro.griffith.authorKennedy, Derek D.
gro.griffith.authorBudi Utama, Alloysius J.


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