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dc.contributor.authorTan, Aimee
dc.contributor.authorPetty, Nicola K.
dc.contributor.authorHocking, Dianna
dc.contributor.authorBennett-Wood, Vicki
dc.contributor.authorWakefield, Matthew
dc.contributor.authorPraszkier, Judyta
dc.contributor.authorTauschek, Marija
dc.contributor.authorYang, Ji
dc.contributor.authorRobins-Browne, Roy
dc.date.accessioned2017-10-30T12:31:52Z
dc.date.available2017-10-30T12:31:52Z
dc.date.issued2015
dc.identifier.issn10985522
dc.identifier.doi10.1128/IAI.02697-14
dc.identifier.urihttp://hdl.handle.net/10072/70162
dc.description.abstractThe evolution of pathogenic bacteria is a multifaceted and complex process, which is strongly influenced by the horizontal acquisition of genetic elements and their subsequent expression in their new hosts. A well-studied example is the RegA regulon of the enteric pathogen Citrobacter rodentium. The RegA regulatory protein is a member of the AraC/XylS superfamily, which coordinates the expression of a gene repertoire that is necessary for full pathogenicity of this murine pathogen. Upon stimulation by an exogenous, gut-associated signal, namely, bicarbonate ions, RegA activates the expression of a series of genes, including virulence factors, such as autotransporters, fimbriae, a dispersin-like protein, and the grlRA operon on the locus of enterocyte effacement pathogenicity island. Interestingly, the genes encoding RegA homologues are distributed across the genus Escherichia, encompassing pathogenic and nonpathogenic subtypes. In this study, we carried out a series of bioinformatic, transcriptional, and functional analyses of the RegA regulons of these bacteria. Our results demonstrated that regA has been horizontally transferred to Escherichia spp. and C. rodentium. Comparative studies of two RegA homologues, namely, those from C. rodentium and E. coli SMS-3-5, a multiresistant environmental strain of E. coli, showed that the two regulators acted similarly in vitro but differed in terms of their abilities to activate the virulence of C. rodentium in vivo, which evidently was due to their differential activation of grlRA. Our data indicate that RegA from C. rodentium has strain-specific adaptations that facilitate infection of its murine host. These findings shed new light on the development of virulence by C. rodentium and on the evolution of virulence-regulatory genes of bacterial pathogens in general.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1384
dc.relation.ispartofpageto1395
dc.relation.ispartofissue4
dc.relation.ispartofjournalInfection and Immunity
dc.relation.ispartofvolume83
dc.rights.retentionY
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchBacteriology
dc.subject.fieldofresearchMicrobial genetics
dc.subject.fieldofresearchAgricultural, veterinary and food sciences
dc.subject.fieldofresearchBiomedical and clinical sciences
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode310701
dc.subject.fieldofresearchcode310704
dc.subject.fieldofresearchcode30
dc.subject.fieldofresearchcode32
dc.titleEvolutionary Adaptation of an AraC-Like Regulatory Protein in Citrobacter rodentium and Escherichia Species
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionVersion of Record (VoR)
gro.rights.copyright© 2015 American Society for Microbiology. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.
gro.hasfulltextFull Text
gro.griffith.authorTan, Aimee


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