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dc.contributor.authorHan, Pingping
dc.contributor.authorIvanovski, Saso
dc.contributor.authorCrawford, Ross
dc.contributor.authorXiao, Yin
dc.date.accessioned2018-06-15T02:17:30Z
dc.date.available2018-06-15T02:17:30Z
dc.date.issued2015
dc.identifier.issn0884-0431
dc.identifier.doi10.1002/jbmr.2445
dc.identifier.urihttp://hdl.handle.net/10072/70163
dc.description.abstractCanonical Wnt signaling is important in tooth development but it is unclear whether it can induce cementogenesis and promote the regeneration of periodontal tissues lost because of disease. Therefore, the aim of this study is to investigate the influence of canonical Wnt signaling enhancers on human periodontal ligament cell (hPDLCs) cementogenic differentiation in vitro and cementum repair in a rat periodontal defect model. Canonical Wnt signaling was induced by (1) local injection of lithium chloride; (2) local injection of sclerostin antibody; and (3) local injection of a lentiviral construct overexpressing ߭catenin. The results showed that the local activation of canonical Wnt signaling resulted in significant new cellular cementum deposition and the formation of well-organized periodontal ligament fibers, which was absent in the control group. In vitro experiments using hPDLCs showed that the Wnt signaling pathway activators significantly increased mineralization, alkaline phosphatase (ALP) activity, and gene and protein expression of the bone and cementum markers osteocalcin (OCN), osteopontin (OPN), cementum protein 1 (CEMP1), and cementum attachment protein (CAP). Our results show that the activation of the canonical Wnt signaling pathway can induce in vivo cementum regeneration and in vitro cementogenic differentiation of hPDLCs.
dc.description.peerreviewedYes
dc.description.publicationstatusYes
dc.languageEnglish
dc.language.isoeng
dc.publisherWiley-Blackwell Publishing
dc.publisher.placeUnited States
dc.relation.ispartofstudentpublicationN
dc.relation.ispartofpagefrom1160
dc.relation.ispartofpageto1174
dc.relation.ispartofissue7
dc.relation.ispartofjournalJournal of Bone and Mineral Research
dc.relation.ispartofvolume30
dc.rights.retentionY
dc.subject.fieldofresearchBiological sciences
dc.subject.fieldofresearchEngineering
dc.subject.fieldofresearchBiomedical and clinical sciences
dc.subject.fieldofresearchPeriodontics
dc.subject.fieldofresearchcode31
dc.subject.fieldofresearchcode40
dc.subject.fieldofresearchcode32
dc.subject.fieldofresearchcode320310
dc.titleActivation of the Canonical Wnt Signaling Pathway Induces Cementum Regeneration
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dc.description.versionAccepted Manuscript (AM)
gro.rights.copyright© 2015 American Society for Bone and Mineral Research. This is the peer reviewed version of the following article: Activation of the canonical Wnt signaling pathway induces cementum regeneration, Journal of Bone and Mineral Research, Volume 30, Issue 7, Pages 1160-1174, 2015, which has been published in final form at https://doi.org/10.1002/jbmr.2445. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html)
gro.hasfulltextFull Text
gro.griffith.authorXiao, Yin


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