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  • Generation of three-dimensional multiple spheroid model of olfactory ensheathing cells using floating liquid marbles

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    Version of Record (VoR)
    Author(s)
    Vadivelu, Raja K
    Ooi, Chin H
    Yao, Rebecca-Qing
    Velasquez, Johana Tello
    Pastrana, Erika
    Diaz-Nido, Javier
    Lim, Filip
    Ekberg, Jenny AK
    Nam-Trung, Nguyen
    St John, James A
    Griffith University Author(s)
    St John, James A.
    Ekberg, Jenny A.
    Nguyen, Nam-Trung
    Vadivelu, Raja
    Ooi, Chin Hong
    Year published
    2015
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    Abstract
    We describe a novel protocol for three-dimensional culturing of olfactory ensheathing cells (OECs), which can be used to understand how OECs interact with other cells in three dimensions. Transplantation of OECs is being trialled for repair of the paralysed spinal cord, with promising but variable results and thus the therapy needs improving. To date, studies of OEC behaviour in a multicellular environment have been hampered by the lack of suitable three-dimensional cell culture models. Here, we exploit the floating liquid marble, a liquid droplet coated with hydrophobic powder and placed on a liquid bath. The presence of ...
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    We describe a novel protocol for three-dimensional culturing of olfactory ensheathing cells (OECs), which can be used to understand how OECs interact with other cells in three dimensions. Transplantation of OECs is being trialled for repair of the paralysed spinal cord, with promising but variable results and thus the therapy needs improving. To date, studies of OEC behaviour in a multicellular environment have been hampered by the lack of suitable three-dimensional cell culture models. Here, we exploit the floating liquid marble, a liquid droplet coated with hydrophobic powder and placed on a liquid bath. The presence of the liquid bath increases the humidity and minimises the effect of evaporation. Floating liquid marbles allow the OECs to freely associate and interact to produce OEC spheroids with uniform shapes and sizes. In contrast, a sessile liquid marble on a solid surface suffers from evaporation and the cells aggregate with irregular shapes. We used floating liquid marbles to co-culture OECs with Schwann cells and astrocytes which formed natural structures without the confines of gels or bounding layers. This protocol can be used to determine how OECs and other cell types associate and interact while forming complex cell structures.
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    Journal Title
    Scientific Reports
    Volume
    5
    DOI
    https://doi.org/10.1038/srep15083
    Copyright Statement
    © The Author(s) 2015. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
    Subject
    Regenerative Medicine (incl. Stem Cells and Tissue Engineering)
    Publication URI
    http://hdl.handle.net/10072/99126
    Collection
    • Journal articles

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