dc.contributor.author | Wang, H | |
dc.contributor.author | Zhang, HM | |
dc.contributor.author | Yin, HJ | |
dc.contributor.author | Wei, MQ | |
dc.contributor.author | Sha, H | |
dc.contributor.author | Liu, TJ | |
dc.contributor.author | Li, YX | |
dc.date.accessioned | 2018-11-06T00:39:56Z | |
dc.date.available | 2018-11-06T00:39:56Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 0268-8921 | |
dc.identifier.doi | 10.1007/s10103-014-1617-1 | |
dc.identifier.uri | http://hdl.handle.net/10072/99265 | |
dc.description.abstract | Photodynamic therapy (PDT) using photosensitized reaction to produce cytotoxicity was used for cancer therapy in recent years. To study the effectiveness of PDT mediated by a novel photosensitizer (PS), DTPP 5-(4′-(2″-dicarboxymethylamino)acetamidophenyl)-10, 15, 20-triphenylporphyrin, on lung cancer A549 cell lines in vitro, DTPP was employed in different concentrations (2, 4, 6, 8, 10, 12, 15, 20, 25, and 30 μg/ml) and combined with 650 nm laser of different power densities (0.6, 1.2, 2.4, 4.8, 7.2, and 9.6 J/cm2) that resulted in obvious inhibition of cell proliferation and apoptosis. Results showed that cell survival rates have a dependent relationship with time and PS concentrations and no significant cytotoxicity was induced by DTPP itself. Apoptosis and cell cycle S arrest were observed; cytoskeleton morphologic observation revealed collapse, sparkling, and shrunken shapes. Apoptosis-related protein caspase-3 overexpression was detected while caspase-9, bcl-2, and cytoskeleton protein beta-catenin were in low levels of expression than the control. Cleavage of beta-catenin by caspase-3 or other proteases from the lysosome might be the main reason for the cytoskeleton collapse as beta-tubulin and actin were at a stable level 12 h after PDT. This paper gives a better understanding of the effectiveness of DTPP-mediated PDT in lung cancer A549 cells both with regard to dosimetry and apoptosis changes. | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Springer | |
dc.relation.ispartofpagefrom | 77 | |
dc.relation.ispartofpageto | 82 | |
dc.relation.ispartofissue | 1 | |
dc.relation.ispartofjournal | Lasers in Medical Science | |
dc.relation.ispartofvolume | 30 | |
dc.subject.fieldofresearch | Biomedical engineering | |
dc.subject.fieldofresearch | Medical devices | |
dc.subject.fieldofresearchcode | 4003 | |
dc.subject.fieldofresearchcode | 400308 | |
dc.title | Combination of a novel photosensitizer DTPP with 650 nm laser results in efficient apoptosis, arresting cell cycle and cytoskeleton protein changes in lung cancer A549 cells | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
gro.hasfulltext | No Full Text | |
gro.griffith.author | Wei, Ming Q. | |