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dc.contributor.authorSharma, Dileep
dc.contributor.authorHamlet, Stephen Mark
dc.contributor.authorPetcu, Eugen Bogdan
dc.contributor.authorIvanovski, Saso
dc.date.accessioned2018-01-18T04:24:35Z
dc.date.available2018-01-18T04:24:35Z
dc.date.issued2016
dc.identifier.issn2045-2322
dc.identifier.doi10.1038/srep20580
dc.identifier.urihttp://hdl.handle.net/10072/99383
dc.description.abstractThe contribution of the local stem cell niche to providing an adequate vascular framework during healing cannot be overemphasized. Bisphosphonates (BPs) are known to have a direct effect on the local vasculature, but their effect on progenitor cell differentiation is unknown. This in vitro study evaluated the effect(s) of various BPs on the differentiation of human placental mesenchymal stem cells (pMSCs) along the endothelial lineage and their subsequent functional and morphogenic capabilities. pMSC multipotency was confirmed by successful differentiation into cells of both the osteogenic and endothelial lineages, as demonstrated by positive Alizarin Red S staining and Ac-LDL uptake. pMSC differentiation in the presence of non-cytotoxic BP concentrations showed that nitrogen containing BPs had a significant inhibitory effect on cell migration and endothelial marker gene expression, as well as compromised endothelial differentiation as demonstrated using von Willebrand factor immunofluorescence staining and tube formation assay. This in vitro study demonstrated that at noncytotoxic levels, nitrogen-containing BPs inhibit differentiation of pMSCs into cells of an endothelial lineage and affect the downstream functional capability of these cells supporting a multi-modal effect of BPs on angiogenesis as pathogenic mechanism contributing to bone healing disorders such as bisphosphonate related osteonecrosis of the jaws (BRONJ).
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherNature Macmillan
dc.relation.ispartofpagefrom20580-1
dc.relation.ispartofpageto20580-11
dc.relation.ispartofjournalScientific Reports
dc.relation.ispartofvolume6
dc.subject.fieldofresearchDentistry not elsewhere classified
dc.subject.fieldofresearchcode110599
dc.titleThe effect of bisphosphonates on the endothelial differentiation of mesenchymal stem cells
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
dcterms.licensehttp://creativecommons.org/licenses/by/4.0/
dc.description.versionVersion of Record (VoR)
gro.facultyGriffith Health, School of Dentistry and Oral Health
gro.rights.copyright© The Author(s).2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
gro.hasfulltextFull Text
gro.griffith.authorHamlet, Stephen
gro.griffith.authorPetcu, Eugen B.
gro.griffith.authorIvanovski, Saso
gro.griffith.authorSharma, Dileep


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