dc.contributor.author | Sharma, Dileep | |
dc.contributor.author | Hamlet, Stephen Mark | |
dc.contributor.author | Petcu, Eugen Bogdan | |
dc.contributor.author | Ivanovski, Saso | |
dc.date.accessioned | 2018-01-18T04:24:35Z | |
dc.date.available | 2018-01-18T04:24:35Z | |
dc.date.issued | 2016 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.doi | 10.1038/srep20580 | |
dc.identifier.uri | http://hdl.handle.net/10072/99383 | |
dc.description.abstract | The contribution of the local stem cell niche to providing an adequate vascular framework during
healing cannot be overemphasized. Bisphosphonates (BPs) are known to have a direct effect on the
local vasculature, but their effect on progenitor cell differentiation is unknown. This in vitro study
evaluated the effect(s) of various BPs on the differentiation of human placental mesenchymal stem cells
(pMSCs) along the endothelial lineage and their subsequent functional and morphogenic capabilities.
pMSC multipotency was confirmed by successful differentiation into cells of both the osteogenic and
endothelial lineages, as demonstrated by positive Alizarin Red S staining and Ac-LDL uptake. pMSC
differentiation in the presence of non-cytotoxic BP concentrations showed that nitrogen containing
BPs had a significant inhibitory effect on cell migration and endothelial marker gene expression,
as well as compromised endothelial differentiation as demonstrated using von Willebrand factor
immunofluorescence staining and tube formation assay. This in vitro study demonstrated that at noncytotoxic
levels, nitrogen-containing BPs inhibit differentiation of pMSCs into cells of an endothelial
lineage and affect the downstream functional capability of these cells supporting a multi-modal effect
of BPs on angiogenesis as pathogenic mechanism contributing to bone healing disorders such as
bisphosphonate related osteonecrosis of the jaws (BRONJ). | |
dc.description.peerreviewed | Yes | |
dc.language | English | |
dc.language.iso | eng | |
dc.publisher | Nature Macmillan | |
dc.relation.ispartofpagefrom | 20580-1 | |
dc.relation.ispartofpageto | 20580-11 | |
dc.relation.ispartofjournal | Scientific Reports | |
dc.relation.ispartofvolume | 6 | |
dc.subject.fieldofresearch | Dentistry not elsewhere classified | |
dc.subject.fieldofresearchcode | 320399 | |
dc.title | The effect of bisphosphonates on the endothelial differentiation of mesenchymal stem cells | |
dc.type | Journal article | |
dc.type.description | C1 - Articles | |
dc.type.code | C - Journal Articles | |
dcterms.license | http://creativecommons.org/licenses/by/4.0/ | |
dc.description.version | Version of Record (VoR) | |
gro.faculty | Griffith Health, School of Dentistry and Oral Health | |
gro.rights.copyright | © The Author(s).2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images
or other third party material in this article are included in the article’s Creative Commons license,
unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license,
users will need to obtain permission from the license holder to reproduce the material. To view a copy of this
license, visit http://creativecommons.org/licenses/by/4.0/ | |
gro.hasfulltext | Full Text | |
gro.griffith.author | Hamlet, Stephen | |