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dc.contributor.authorSalajegheh, Ali
dc.contributor.authorVosgha, Haleh
dc.contributor.authorRahman, Md Atiqur
dc.contributor.authorAmin, Moein
dc.contributor.authorSmith, Robert Anthony
dc.contributor.authorLam, Alfred King-Yin
dc.date.accessioned2018-07-27T03:22:32Z
dc.date.available2018-07-27T03:22:32Z
dc.date.issued2016
dc.identifier.issn0046-8177
dc.identifier.doi10.1016/j.humpath.2015.12.018
dc.identifier.urihttp://hdl.handle.net/10072/99531
dc.description.abstractMicroRNA-126 (miR-126) expression has been shown to be associated with angiogenesis. The aim of the current study is to evaluate the functional roles of miR-126 in dysregulation of VEGF expression and cancer progression in thyroid carcinomas. The expression of VEGF-A and miR-126 were measured in 101 thyroid carcinomas tissues (including 51 conventional papillary thyroid carcinoma, 37 follicular variant of papillary thyroid carcinoma, and 13 undifferentiated thyroid carcinomas), 13 matched lymph nodes with metastatic thyroid carcinoma, 21 benign thyroid tissues, and 5 thyroid carcinoma cell lines (both papillary and undifferentiated carcinomas). Then, exogenous miR-126 was transfected, and the expressions of VEGF-A were determined (Western blot technique). Proliferation assay, cell cycle analysis, and apoptosis assays were used to evaluate the role of miR-126 in these events. Significant underexpression of miR-126 levels in thyroid cancer tissues and cell lines was detected using real-time polymerase chain reaction. Introducing exogenous miR-126 into the cancer cell lines resulted in a significant reduction of VEGF-A protein expression. Marked inhibition in proliferation, cell cycle arrest in G0-G1, and promotion of total apoptosis were also noted. The modulatory role of miR-126 on expression of VEGF-A and its tumor suppressive roles were demonstrated for the first time in thyroid cancer. The current experiments provided specific information on the functional consequences of VEGF manipulation via microRNA on cancer.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom75
dc.relation.ispartofpageto85
dc.relation.ispartofjournalHuman Pathology
dc.relation.ispartofvolume51
dc.subject.fieldofresearchClinical sciences
dc.subject.fieldofresearchClinical sciences not elsewhere classified
dc.subject.fieldofresearchcode3202
dc.subject.fieldofresearchcode320299
dc.titleInteractive role of miR-126 on VEGF-A and progression of papillary and undifferentiated thyroid carcinoma
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.facultyGriffith Health, School of Medicine
gro.hasfulltextNo Full Text
gro.griffith.authorLam, Alfred K.
gro.griffith.authorAriana, Armin S.


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