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dc.contributor.authorSinclair, Holly A
dc.contributor.authorHeney, Claire
dc.contributor.authorSidjabat, Hanna E
dc.contributor.authorGeorge, Narelle M
dc.contributor.authorBergh, Haakon
dc.contributor.authorAnuj, Snehal N
dc.contributor.authorNimmo, Graeme R
dc.contributor.authorPaterson, David L
dc.date.accessioned2018-11-05T05:46:32Z
dc.date.available2018-11-05T05:46:32Z
dc.date.issued2016
dc.identifier.issn0732-8893
dc.identifier.doi10.1016/j.diagmicrobio.2016.02.005
dc.identifier.urihttp://hdl.handle.net/10072/99889
dc.description.abstractInfection caused by Aeromonas spp. ranges from superficial wound infection to life-threatening septicemia. Carbapenem resistance due to metallo-beta-lactamase, CphA encoded by the cphA gene, is a significant problem. This study defines Aeromonas spp. causing clinical disease in Queensland, Australia. Phenotypic tests for carbapenemase detection were assessed. One hundred Aeromonas isolates from blood (22), wound (46), sterile sites (11), stool (18), eye (2), and sputum (1) were characterized by rpoB and gyrB sequencing. Meropenem susceptibility by VITEK2, disk diffusion, and E-test MIC were determined. Carbapenemase production was assessed by Carba NP test and cphA by PCR. Gene sequencing identified isolates as Aeromonas dhakensis (39), Aeromonas veronii (21), Aeromonas hydrophila (20), Aeromonas caviae (14), Aeromonas jandaei (4), Aeromonas bestiarum (1), and Aeromonas sanarellii (1). Disk diffusion and E-test failed to detect resistance in isolates with presence of cphA. Carba NP was performed with 97.4% sensitivity and 95.7% specificity. Carbapenem resistance gene cphA was detected in A. veronii (21; 100%), A. hydrophila (18; 90%), A. dhakensis (34; 87.2%), A. jandaei (3; 75%), and A. bestiarum (1; 100%) but not A. caviae. We found that A. dhakensis was the predominant species, a previously unrecognized pathogen in this region.
dc.description.peerreviewedYes
dc.languageEnglish
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofpagefrom98
dc.relation.ispartofpageto101
dc.relation.ispartofissue1
dc.relation.ispartofjournalDiagnostic Microbiology and Infectious Disease
dc.relation.ispartofvolume85
dc.subject.fieldofresearchMicrobiology not elsewhere classified
dc.subject.fieldofresearchMicrobiology
dc.subject.fieldofresearchClinical Sciences
dc.subject.fieldofresearchMedical Microbiology
dc.subject.fieldofresearchcode060599
dc.subject.fieldofresearchcode0605
dc.subject.fieldofresearchcode1103
dc.subject.fieldofresearchcode1108
dc.titleGenotypic and phenotypic identification of Aeromonas species and CphA-mediated carbapenem resistance in Queensland, Australia
dc.typeJournal article
dc.type.descriptionC1 - Articles
dc.type.codeC - Journal Articles
gro.hasfulltextNo Full Text
gro.griffith.authorNimmo, Graeme R.
gro.griffith.authorSidjabat, Hanna E.


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