In Situ Growth Rate of Microcystis in a Hypertrophic Lake: RNA/DNA Ratio as an Indicator

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Li, Ming
Xiao, Man
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2014
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Abstract

The DNA content was employed as an indicator of algae biomass to improve the cellular RNA content method to estimate the in situ growth rate of Microcystis in a hypertrophic lake (Lake Taihu, China). Microcystis aeruginosa was cultured in BG-11 and M-11 mediums, respectively, and a significant relationship between cell density and DNA concentration of Microcystis was found. Then a significant logarithmic relationship between Microcystis growth rate and RNA/DNA ratio was established to measure growth rate of Microcystis by analysis of RNA and DNA content. The growth rates calculated by RNA/DNA method were in good agreement with that calculated by the changes of cell density in the whole water column respected with time. The RNA/DNA method was applied in Lake Taihu in summer (1st July) and autumn (1st October), respectively. In summer, the maximum growth rate was 0.361 day-1 and others were from 0.04 to 0.34 day-1 with an average value of 0.12, four values were below zero; In autumn, the maximum growth rate was 0.111 day-1 and most values were below zero. Our results also demonstrated that the growth rate of Microcystis was higher inshore than offshore. © by PSP.

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Fresenius Environmental Bulletin
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23
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4
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Chemical sciences
Environmental sciences
Biological sciences
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Life Sciences & Biomedicine
Environmental Sciences & Ecology
Microcystis
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Li, M; Xiao, M, In Situ Growth Rate of Microcystis in a Hypertrophic Lake: RNA/DNA Ratio as an Indicator, Fresenius Environmental BulletinN, 2014, 23 (4), pp. 1100-1106
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