Campylobacter fetus is recognized as an important animal and human pathogen. Chemotaxis and motility have been identified as important virulence factors associated with C. jejuni colonisation. This study established that chemoreceptor and chemotaxis signal transduction cascade genes of C. fetus subsp. fetus 82-40 showed high level of similarity to that in C. jejuni and appears to include sixteen diverse transducer-like protein (tlp) genes that are similar to nine of the twelve tlp genes in the C. jejuni NCTC 11168 with a percent identity ranging from 15 to 50%. Sixteen putative C. fetus 82-40 tlp genes belong to three classes: A, B, and C, as well as an aerotaxis gene, based on their predicted structure. C. fetus subsp. fetus 82-40 chemoreceptor and chemotaxis signal transduction pathway genes have close phylogenetic relationship of chemotaxis genes between Campylobacteraceae and Helicobacteraceae.