Salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosus
File version
Version of Record (VoR)
Author(s)
Camara Sete, Manuela Rubim
Freitas-Fernandes, Liana Bastos
Sztajnbok, Flavio Roberto
Figueredo, Carlos Marcelo
Valente, Ana Paula
da Silva Fidalgo, Tatiana Kelly
Silva, Fernanda de Brito
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)
Date
Size
File type(s)
Location
Abstract
The aim of this study was to characterize the salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosus (jSLE). A total of 24 adolescents with jSLE (15.92 ± 2.06 years) and 12 systemically healthy controls (15.25 ± 2.7 years) were included in the study. Participants underwent rheumatologic testing and periodontal examination, with the recording of plaque index (PI), probing depth (PD), clinical attachment level (CAL), and bleeding on probing index (BPI). Unstimulated whole saliva was collected from both groups and stored at -80 ºC. The salivary proton nuclear magnetic resonance (1H-NMR) spectra were acquired in a spectrometer operating at 500 MHz. Partial least squared discriminant analysis (PLS-DA) and orthogonal PLS-DA (O-PLS-DA) were used for statistical analysis. Mean CAL and PI were significantly increased in the group with jSLE (p < 0.01). Patients with jSLE presented a significantly different salivary metabolic profile (accuracy = 0.54; R2 = 0.86; Q2 = -0.293), significantly higher salivary levels of N-acetyl sugars, and significantly reduced levels of phenylalanine, glycine, taurine, hydroxybutyrate, and valerate compared with healthy controls (p < 0.05). It is suggested that the salivary metabolomic profile analyzed by 1H NMR in patients with jSLE presents a different fingerprint that the systemically healthy subjects. Integrating the variation of metabolites with the identification of the metabolic pathways involved seems to provide a better understanding of the influence of systemic disease on salivary metabolites.
Journal Title
Brazilian Oral Research
Conference Title
Book Title
Edition
Volume
36
Issue
Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement
This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Item Access Status
Note
Access the data
Related item(s)
Subject
Medical biochemistry and metabolomics
Oral medicine and pathology
Science & Technology
Life Sciences & Biomedicine
Dentistry, Oral Surgery & Medicine
Gingivitis
Metabolomics
Persistent link to this record
Citation
Agostinho Bragard, LC; Camara Sete, MR; Freitas-Fernandes, LB; Sztajnbok, FR; Figueredo, CM; Valente, AP; da Silva Fidalgo, TK; Silva, FDB, Salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosus, Brazilian Oral Research, 2022, 36, pp. e0128