Salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosus

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Agostinho Bragard, Loreley Carlos
Camara Sete, Manuela Rubim
Freitas-Fernandes, Liana Bastos
Sztajnbok, Flavio Roberto
Figueredo, Carlos Marcelo
Valente, Ana Paula
da Silva Fidalgo, Tatiana Kelly
Silva, Fernanda de Brito
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2022
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Abstract

The aim of this study was to characterize the salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosus (jSLE). A total of 24 adolescents with jSLE (15.92 ± 2.06 years) and 12 systemically healthy controls (15.25 ± 2.7 years) were included in the study. Participants underwent rheumatologic testing and periodontal examination, with the recording of plaque index (PI), probing depth (PD), clinical attachment level (CAL), and bleeding on probing index (BPI). Unstimulated whole saliva was collected from both groups and stored at -80 ºC. The salivary proton nuclear magnetic resonance (1H-NMR) spectra were acquired in a spectrometer operating at 500 MHz. Partial least squared discriminant analysis (PLS-DA) and orthogonal PLS-DA (O-PLS-DA) were used for statistical analysis. Mean CAL and PI were significantly increased in the group with jSLE (p < 0.01). Patients with jSLE presented a significantly different salivary metabolic profile (accuracy = 0.54; R2 = 0.86; Q2 = -0.293), significantly higher salivary levels of N-acetyl sugars, and significantly reduced levels of phenylalanine, glycine, taurine, hydroxybutyrate, and valerate compared with healthy controls (p < 0.05). It is suggested that the salivary metabolomic profile analyzed by 1H NMR in patients with jSLE presents a different fingerprint that the systemically healthy subjects. Integrating the variation of metabolites with the identification of the metabolic pathways involved seems to provide a better understanding of the influence of systemic disease on salivary metabolites.

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Brazilian Oral Research

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36

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This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Medical biochemistry and metabolomics

Oral medicine and pathology

Science & Technology

Life Sciences & Biomedicine

Dentistry, Oral Surgery & Medicine

Gingivitis

Metabolomics

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Agostinho Bragard, LC; Camara Sete, MR; Freitas-Fernandes, LB; Sztajnbok, FR; Figueredo, CM; Valente, AP; da Silva Fidalgo, TK; Silva, FDB, Salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosus, Brazilian Oral Research, 2022, 36, pp. e0128

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