Bicycle infrastructure is being constructed in Australia to encourage safer and faster cycling trips. However, there has been limited evaluation of the impact of infrastructure investments. This study examined the behavioural impact of a new segment of a dedicated bikeway (Veloway 1 [V1] Stage C) that links southern suburbs with Brisbane city centre. The V1 Stage C opened in June, 2013. Cyclists who used a pre-existing shared path that links southern suburbs with the city centre completed an intercept survey pre- and post-V1 Stage C opening. Cyclists who used the V1 Stage C after it opened completed the same survey at the same time post-V1 Stage C opening. Survey data were complemented by GPS bicycle count data from cyclists riding on the main cycle routes into the city centre from southern suburbs: the V1 Stage C, the pre-existing shared path and a major arterial road. Survey data showed that pre- to post-V1 Stage C opening, average bicycle trip distance of cyclists using the shared path decreased (p=0.002), and the main catchment area of that path narrowed to suburbs to the west of it (p<0.001). Compared to cyclists using the shared path post-V1 Stage C opening, those using the V1 were travelling longer distances (p=0.02) and were more committed to making their trip by bicycle (p=0.001). The GPS bicycle counts increased monthly on the V1 Stage C after it opened (p<0.002). GPS bicycle counts on the alternative routes did not change (p=0.84). There were greater increases in monthly counts of cyclists approaching Brisbane from the south post-V1 Stage C opening than pre-opening. The findings suggest that veloways like the V1 can attract cyclists travelling from outer suburbs into a city centre.