Individual differences in skill level during sport-related motor tasks, such as golf putting, can be related to not only performance, but also patterns in psychophysiological activity. The present study examined the similarities and differences in cardiac and respiratory activity among elite, experienced, and novice golfers. Participants attempted flat putts 2.4 m from the hole. Performance was better in elite and experienced golfers than in novice golfers. Compared to novice golfers, the experienced and elite golfers showed a pronounced phasic deceleration in heart rate immediately prior to the putt, greater heart rate variability in the very low frequency band, and a greater tendency to show a respiratory pattern of exhaling immediately prior to the putt. The psychophysiological patterns may be related to differences in attentional processes or task familiarity between the groups. The implications of the results for the assessment and training of athletes in precision sports is discussed.