Cancer biomarker profiling using nanozyme containing iron oxide loaded with gold particles
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Shiddiky, Muhammad JA
Kim, Tak H
Brown, Christopher L
Yamauchi, Yusuke
Cock, Ian E
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Abstract
Nanozymes are nanomaterials with intrinsic magnetism and superparamagnetic properties. In the presence of an external magnet, nanozyme particles aggregate and redisperse without a foreign attraction. We evaluated the performances of nanozyme by changing the biosensing platforms and substituting other biological variants for a complete cancer assay detection. We investigated the expression of morphological variants in the transmission of signals using an electrochemical method. The signal responses, including signal enhancement with the nanozyme (Au-Fe2O3), showed a wide capturing range (greater than 80%, from 102 to 105 cells ml−1 in phosphate-buffered saline buffer, pH 7.4). The platform showed a fast response time within a dynamic range of 10–105 cells ml−1 for the investigated T47D cancer cell line. We also obtained higher responses for anti-HER2 (human epidermal receptor 2)/streptavidin interface as the biosensing electrode in the presence of T47D cancer cells. The positive assay produced a sixfold increase in current output compared to the negative target or negative biological variant. We calculated the limit of detection at 0.4 U ml−1, and of quantitation at 4 U ml−1 (units per millilitre). However, blood volume amounts in clinical settings may constrain diagnosis and increase detection limit value significantly.
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Journal of the Royal Society Interface
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17
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167
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© 2020 Royal Society. This is the author-manuscript version of this paper. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal website for access to the definitive, published version.
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Biological sciences
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
amperometry detection
cancer biomarkers
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Akpe, V; Shiddiky, MJA; Kim, TH; Brown, CL; Yamauchi, Y; Cock, IE, Cancer biomarker profiling using nanozyme containing iron oxide loaded with gold particles, Journal of the Royal Society Interface, 2020, 17 (167)