Detection of regional DNA methylation using DNA-graphene affinity interactions
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Gopalan, Vinod
Yadav, Sharda
Islam, Md Nazmul
Eftekhari, Ehsan
Li, Qin
Carrascosa, Laura G
Nam-Trung, Nguyen
Lam, Alfred K
Shiddiky, Muhammad JA
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Abstract
We report a new method for the detection of regional DNA methylation using base-dependent affinity interaction (i.e., adsorption) of DNA with graphene. Due to the strongest adsorption affinity of guanine bases towards graphene, bisulfite-treated guanine-enriched methylated DNA leads to a larger amount of the adsorbed DNA on the graphene-modified electrodes in comparison to the adenine-enriched unmethylated DNA. The level of the methylation is quantified by monitoring the differential pulse voltammetric current as a function of the adsorbed DNA. The assay is sensitive to distinguish methylated and unmethylated DNA sequences at single CpG resolution by differentiating changes in DNA methylation as low as 5%. Furthermore, this method has been used to detect methylation levels in a collection of DNA samples taken from oesophageal cancer tissues.
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Biosensors and Bioelectronics
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87
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© 2017, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence, which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited.
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Analytical chemistry
Analytical chemistry not elsewhere classified
Biomedical engineering
Nanotechnology