Purpose: Sport-science research consistently contains repeated measures and imbalanced data sets. This study calls for further adoption of mixed models when analyzing longitudinal sport-science data sets. Mixed models were used to understand whether the level of competition affected the intensity of women’s rugby league match play. Methods: A total of 472 observations were used to compare the mean speed of female rugby league athletes recorded during club-, state-, and international-level competition. As athletes featured in all 3 levels of competition and there were multiple matches within each competition (ie, repeated measures), the authors demonstrated that mixed models are the appropriate statistical approach for these data. Results: The authors determined that if a repeated-measures analysis of variance (ANOVA) were used for the statistical analysis in the present study, at least 48.7% of the data would have been omitted to meet ANOVA assumptions. Using a mixed model, the authors determined that mean speed recorded during Trans-Tasman Test matches was 73.4 m·min−1, while the mean speeds for National Rugby League Women and State of Origin matches were 77.6 and 81.6 m·min−1, respectively. Random effects of team, athlete, and match all accounted for variations in mean speed, which otherwise could have concealed the main effects of position and level of competition had less flexible ANOVAs been used. Conclusion: These data clearly demonstrate the appropriateness of applying mixed models to typical data sets acquired in the professional sport setting. Mixed models should be more readily used within sport science, especially in observational, longitudinal data sets such as movement pattern analyses.