Diagnostic assays and sampling protocols for the detection of Batrachochytrium dendrobatidis.

No Thumbnail Available
File version
Author(s)
Hyatt, A.
Boyle, D.
Olsen, V.
Boyle, D.
Berger, L.
Obendorf, D.
Dalton, A.
Kriger, Kerry
Hero, Jean-Marc
Hines, H.
Phillott, R.
Campbell, R.
Marantelli, C.
Gleason, F.
Colling, A.
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)
Date
2007
Size
File type(s)
Location
License
Abstract

Batrachochytrium dendrobatidis is a fungus belonging to the Phylum Chytridiomycota, Class Chytridiomycetes, Order Chytridiales, and is the highly infectious aetiological agent responsible for a potentially fatal disease, chytridiomycosis, which is currently decimating many of the world's amphibian populations. The fungus infects 2 amphibian orders (Anura and Caudata), 14 families and at least 200 species and is responsible for at least 1 species extinction. Whilst the origin of the agent and routes of transmission are being debated, it has been recognised that successful management of the disease will require effective sampling regimes and detection assays. We have developed a range of unique sampling protocols together with diagnostic assays for the detection of B. dendrobatidis in both living and deceased tadpoles and adults. Here, we formally present our data and discuss them in respect to assay sensitivity, specificity, repeatability and reproducibility. We suggest that compliance with the recommended protocols will avoid the generation of spurious results, thereby providing the international scientific and regulatory community with a set of validated procedures which will assist in the successful management of chytridiomycosis in the future.

Journal Title

Diseases of Aquatic Organisms

Conference Title
Book Title
Edition
Volume

73

Issue
Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement
Item Access Status
Note
Access the data
Related item(s)
Subject

Biological sciences

Agricultural, veterinary and food sciences

Persistent link to this record
Citation
Collections