Association mapping (AM), also known as genome-wide association studies (GWAS), is increasingly being employed in crop plants for the identification of QTL/genes and marker–trait associations (MTAs) in natural populations. Large numbers of such associations have been identified for variety of traits in different crop plants. However, not many of these associations have been used practically in the crop improvement program due to lack of validation. Although there are different ways through which the results of AM/GWAS could be validated, the best approach is to develop a biparental population for the trait of interest. An overview of the steps involved in the validation of results of AM using biparental mapping population in plants is provided in this chapter.