Characterization of regeneration initiating cells during Xenopus laevis tail regeneration

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Sindelka, Radek
Naraine, Ravindra
Abaffy, Pavel
Zucha, Daniel
Kraus, Daniel
Netusil, Jiri
Smetana, Karel
Lacina, Lukas
Endaya, Berwini Beduya
Neuzil, Jiri
Psenicka, Martin
Kubista, Mikael
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2024
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Abstract

Background Embryos are regeneration and wound healing masters. They rapidly close wounds and scarlessly remodel and regenerate injured tissue. Regeneration has been extensively studied in many animal models using new tools such as single-cell analysis. However, until now, they have been based primarily on experiments assessing from 1 day post injury.

Results In this paper, we reveal that critical steps initiating regeneration occur within hours after injury. We discovered the regeneration initiating cells (RICs) using single-cell and spatial transcriptomics of the regenerating Xenopus laevis tail. RICs are formed transiently from the basal epidermal cells, and their expression signature suggests they are important for modifying the surrounding extracellular matrix thus regulating development. The absence or deregulation of RICs leads to excessive extracellular matrix deposition and defective regeneration.

Conclusion RICs represent a newly discovered transient cell state involved in the initiation of the regeneration process.

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Genome Biology

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25

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© The Author(s) 2024. This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Sindelka, R; Naraine, R; Abaffy, P; Zucha, D; Kraus, D; Netusil, J; Smetana, K; Lacina, L; Endaya, BB; Neuzil, J; Psenicka, M; Kubista, M, Characterization of regeneration initiating cells during Xenopus laevis tail regeneration, Genome Biology, 2024, 25, pp. 251

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