For the first time, the ionic polymer metal composite (IPMC) actuator is integrated with a radio frequency identification (RFID) tag antenna for achieving frequency reconfiguration in the ultrahigh-frequency (UHF) band. Here, the IPMC movable flap serves as an actuator that can effectively tune the tag resonant frequency. By applying a two-layer-crenelated structure and employing a surface patterned electrode for restraining back relaxation, the deflection of the IPMC actuator can be significantly improved. The IPMC actuator can move in two directions, enabling two-degree frequency tuning. Numerical and experimental data confirm that the tip displacement of the IPMC actuator can be enhanced up to 266% with the use of the two-layer-crenelated structure. The IPMC actuator allows the resonant frequency of the tag antenna to be tuned back by as much as 35 MHz, after deviating due to placing on an unintended object. UHF RFID application is also performed using a portable commercial RFID reader. Good frequency reconfiguration and broad tuning range, along with far read distances, have been achieved with our tunable UHF RFID tag antenna.